The question arises from seeing photolyases being advertised for skin DNA-repair in living people
Dear all, I'm conducting the revisions of some of my manuscript, with is the impact of the combination of Aspirin and a fruit juice in colorectal cancer cells. Two of the reviewers have asked to...
16 February 2021 3,141 3 View
We need to calculate multiphase stream of the one substance in the air atmosphere (this is why we using interFoam), but also we need to get heat transfer data between the substance, solid obstacle...
11 January 2021 872 2 View
I'facing problem while doing HPLC analysis due to negative peak was eluted which was corresponding to the principle peak RT. I tried to remove, with different solvents, but no use. i want to know...
26 November 2020 1,960 5 View
Dear Research scholars, I was doing my MTech thesis on pendulum based TMD and I was having trouble defining the link properties. I even followed the tutorials put on CSI website. still, I wasn't...
11 November 2020 8,309 11 View
Greetings, I'm currently using the ANSYS 2020R1 crack version. I used this software for at least one month smoothly. But in the last few days, I'm facing this problem. I'm tired to solve this...
09 November 2020 6,431 2 View
I am trying to create polygon shapefile with land use from Second military survey using Arcmap. However, I really have hard time with reading the map, more correctly with boundaries between...
07 November 2020 6,018 3 View
In the pulp and paper company that I work in, samples from paper, water and pulp from the paper machine gave information about existing spores of Presumptive Bacillus cereus. Our paper is used...
03 November 2020 1,726 10 View
I have certain cells that are contaminant cells in a suspension of cells. I found a buffer that selectively removes them, but a lot of protocols don't specify what volume one should use for that...
03 November 2020 6,330 3 View
I am trying to look at the expression of c-fos nuclear protein in mice brain using WB technique. Can anyone suggest a protocol to extract the proteins? Better harsh homogenate before sonication?...
27 October 2020 5,272 2 View
I have sperm cells extracted from epididymis of a mouse. I would like to check and quantify their purity. My first idea is to use an intercalating dye like Hoechst and then see what fraction of...
25 October 2020 8,600 1 View
Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?
03 March 2021 1,763 2 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity
03 March 2021 8,890 1 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
01 March 2021 3,355 3 View
Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!
28 February 2021 3,951 2 View
I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...
27 February 2021 356 6 View
Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...
27 February 2021 5,028 2 View
Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...
26 February 2021 5,029 2 View
Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...
25 February 2021 6,969 3 View