How I could make a scattered graph for single cell analysis like this?

I want to know which software that could make a scattered graph like that?

07 August 2018 6,754 2 View

What is the cell tracker dye with the highest fluorescence?

We culture cells on a microchip with a membrane that has autofluorescence and after few hours the stained cells start fading I am searching for a cell tracker dye with strong intensity to make it...

09 October 2017 9,174 3 View

How could I measure the permeability of a membrane or the pore size?

08 September 2017 7,643 1 View

What is the best condition for MD MB231 to migrate?

I am doing cell migration assay for MD MB231 on a microchip using collagen to coat the surface, cells look healthy and attached to the collagen but they don't show migration after 12 hrs. How...

08 September 2017 5,584 1 View

What is the effect of the base on tetrazine?

I want to make coupling reaction between a compound with carboxylic group and the aminethat have tetrazine. I used HATU and DIPEA for the coupling for overnight. I get three spots may be the...

05 June 2017 9,431 2 View

What is the stability of tetrazine in basic medium and acidic medium?

I want to know if the tetrazine is table or not in acidic or basic medium.

04 May 2017 903 0 View

How could I purify PEG conjugated Products?

I will react PEG-NH2 with a small compound that has carboxylic acid group using a coupling agent. But I just wondering How could Purify the product?

03 April 2017 2,811 2 View

How to make a chain of magnetic microbeads?

I want to make a chain from magnetic microbeads

11 December 2016 805 3 View

What is the proximity effct in organic chemistry?

Proximity effect definition and example

08 September 2016 3,078 0 View

What is the mechanism of action of Carboplatin as anticancer?

I want to know the detailed mechanism of cis platin and carboplatin with references please

08 September 2016 1,994 2 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View