How could I sterilise hydrogel made of silk fibroin?

More Bibhas Bhunia's questions See All

Does ADRC controller provide sufficient robustness to external disturbances?

Actually, I want to compare robustness between ADRC & H infinity controller. ADRC is a sort of model free control whereas H infinity is model based. Suppose, a system is prone to cyber attack....

04 May 2021 3,776 2 View

Which one is having better accuracy in terms of reconstruction?

I want to compare accuracy among these four methods in terms of reconstruction. These four methods are mentioned below: 1. EMD (empirical mode decomposition) 2. DMD (dynamic mode decomposition) 3....

02 May 2021 3,894 9 View

How to export velocity data from MATLAB to TecPlot to plot the streamlines?

Streamline plots in MATLAB shows streamline only in the direction of the flow. But tecplot has options to plot in both direction. But Cant export data to tecplot.

09 October 2020 3,602 2 View

Is there any good journals which publish fuzzy group papers without fee?

Please provide some free SCI/SCIE/SCOPUS indexed journal for fuzzy group.

30 December 2019 6,138 1 View

Error in CHEMKIN file during flamelet generation. it says ""Unexpected word, , in CHEMKIN file, at line. no. 1"". How to get rid of this one?

Any idea is deeply appreciated.

17 September 2019 2,147 2 View

Can we calculate the reaction rate while using the non-premixed combustion model (which is based on mixture fraction theory) in Ansys-fluent?

For finite rate chemistry, the reaction rate can easily be calculated. However, the mixture fraction theory simplifies a combustion reaction into a mixing phenomenon, and the difficulties...

13 September 2019 1,970 3 View

How to calculate Genotypic and Phenotypic Correlations?

I want to correlate gene expression values of a same gene across different genotypes to check wheather the gene is closely correlate or least correlate or negatively correlate with the genotypes?...

09 June 2019 9,332 4 View

Why the relaxed structure got distorted ??

Dear SIESTA users, I am using SIESTA (4.1-b3) code for the relaxation of graphene-metal oxide interface structure. It has been seen that after few 'vc-relaxation' steps, some atoms of the...

06 February 2019 4,516 5 View

Is it possible to make an interface between hexagonal and tetragonal lattice structures ?

I want to create an interface (stack) between Graphene and TiO2 unitcells. As we know that the graphene has hexagonal lattice structure and TiO2 has tetragonal one, is it possible to make the...

11 December 2018 7,254 2 View

Can anyone please help me to solve the problem regarding transiesta run at higher bias points ??

Dear Experts, I am using transiesta code to get the I-V characteristics of a 2D structure (defected AGNR sheet). All the calculations (electrode, transiesta and tbtrans) run perfectly for some...

21 May 2018 5,138 4 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View