How could I quantify dopamine receptor 2 protein expression in human blood?

20 May 2014 0 6K Report

I would like to investigate the dopamine receptor 2 expression (protein and mRNA) in human blood. I was wondering if I could get some advice on which method to use.

Badges
Science topic

More Laetitia Schwab's questions See All

How to measure the concentration of monoclonal antibodieswithout nanodrop ?

Dear all, I'm going to radiolabel antibodies. So first of all, I'll remove excipients using the centrifugation method with amicon. Once I'm done, how do I calculate/measure the concentration of...

14 July 2024 3,106 3 View

How to perform centrifugal filtration/ultrafiltration to pembrolizumab?

Dear researchers, I would like to radiolabel pembrolizumab with zirconium-89 but first I need to remove excipients by ultrafiltration or concentration. I'm thinking of doing it using...

24 April 2024 8,163 0 View

Remove excipient from Ketruda ?

Dear all, I would like to radiolabel pembrolizumab with a metal and would like to remove the excipient. I read in some paper that I can use centrifugation but have no idea of how it is supposed to...

05 February 2024 5,065 0 View

How to do you proceed with conjugaison of monoclonal antibodies ?

Dear Researchers, I'll be working on conjugaison reactions with monoclonal antibodies, have somebody already done that before ? This is my first time using monoclonal antibodies ? What about...

22 June 2023 6,624 3 View

Can we use PD10 column for the purification of conjugate radiolabeled antibodies ?

Hi everyone, I'll work on zirconium-89 radiolabeled antibodies and would like to have some help for conjugate antibodies purification. I'm thinking about using PD10 column, is it a good idea ?

12 April 2023 5,302 6 View

strange derivative curves with PCR HRM?

Dears collegues, I obtained strange derivative curves (difference between samples) of my PCR HRM : the peacks are angular. It's a 150bp amplification of ADN from a bacterial communinities with...

06 April 2023 2,304 0 View

What are good electroporation settings for SNU1079/SNU1196/SNU478 or HUCCT1 cells?

Hello everyone, Does anybody have experience with electroporation of Cholangiocarcinoma cell lines? I was trying to find published settings for a pilot experiment involving the electroporation of...

13 March 2023 1,953 0 View

What type of eosin should I use to stain tiny organs before embedding ?

Hello all, I've been trying to stain tiny organs with eosin yellowish acid 87 in ethanol 70% before paraffin embedding but the staining does not last after the dehydration process. I've read some...

15 July 2021 5,952 5 View

How to personalize math learning according to profiles?

Hello, I am interested in the personalization of learning based on profiles, more specifically in mathematics. Do you know any relevant references? Thank you

11 October 2020 4,691 0 View

Amplification in the same NRT after a second or third qPCR?

Hi everyone, If somebody can explain to me what's happening to my samples it would be great ! I'm currently testing a RT-qPCR for three primers, one of wich can amplificate genomic DNA if...

23 August 2020 5,145 3 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View