How to personalize math learning according to profiles?

Hello, I am interested in the personalization of learning based on profiles, more specifically in mathematics. Do you know any relevant references? Thank you

11 October 2020 4,473 0 View

Amplification in the same NRT after a second or third qPCR?

Hi everyone, If somebody can explain to me what's happening to my samples it would be great ! I'm currently testing a RT-qPCR for three primers, one of wich can amplificate genomic DNA if...

23 August 2020 5,060 3 View

Has anyone dosed IL22RA2 (IL-22BP) by elisa?

and if so, does bound IL-22 interfere with IL-22BP detection?

01 March 2016 6,650 2 View

Could anyone suggest to me a method to dissolve Sulpiride for mice treatment (to a dose of 30mg/kg)?

Hi, I am trying to find a way to dissolve and dilute 100mg of Sulpiride for the treatment of mice at a dose of 30mg/kg and a volume of 10ml/kg. Thanks

23 November 2014 9,476 2 View

How do I calculate the RQ with two different housekeeping genes for real-time PCR data?

I have done the real-time qPCR and have the Ct for my target gene and two reference genes, but I don't know how to integrate the two reference genes in the calculation of the relative expression...

24 February 2014 6,911 2 View

No title

23 February 2021 4,014 3 View

Can anyone help or comment on solubilising chlorobutanol in aqueous injections in minimum time possible?

Please suggest solubilisation of chlorobutanol in injections. Tried pH and high temperature. But the issue is of assay drop. If anyone can suggest any solution. Its a commonly used excipient for...

21 February 2021 7,397 1 View

XRPD Quantification - Building a calibration curve of drug excipient?

I am aiming to utilise XRPD to quantify a drug excipient within a drug product and have been having a little trouble building the calibration curve to allow for this quantification up until this...

10 February 2021 6,145 3 View

What is best technique to quantify necrotic cell death of specific cell populations?

I currently using FACS (Aurora Cytek) to quantify necrotic Gr1+ cell death from the spleens of immune competent mice. I target Gr1+ cells in the spleens of tumor-bearing mice using a photodynamic...

08 February 2021 733 6 View

Does viral RNA extraction from nasopharyngeal swabs require pre-centrifugation with supernatant collection?

06 February 2021 323 3 View

How to Remove DNA From a Sample Without Rupturing Yeast?

I transformed a plasmid into yeast and selected for transformants by growing them in a selective media (liquid, not agar plate). My experiment requires that I continually grow/ split these...

01 February 2021 9,936 2 View

What is the minimum centrifugation RPM and minimum time for TiO2 nanoparticle synthesis from plant extract?

In my laboratory, I have an analog centrifuge (does not show real RPM value). Its' brochure indicates the range is 300-3000 RPM. The regulator is marked from 1 to 10. Is there any way to determine...

01 February 2021 2,061 4 View

Does anyone have experience with NCI H716 cell line?

Hello! I am working with NCI H716 cells and I am struggling a bit trying to "wash" the cells since they are producing significant debris. I have centrifugated and resuspended them in their media...

28 January 2021 6,421 1 View

What time and speed should the centrifuge be set at in order for cell debris and clumped proteins to form a pellet?

- The Eppendorf tube was then placed in a microcentrifuge. Notice how it is balanced with another tube placed opposite it in the centrifuge drum to ensure smooth rotation. Other information...

24 January 2021 2,547 3 View

How to calculate an endotoxin limit with a drug concentration in vp/mL?

Hello, I am trying to validate an endotoxin method for an excipient. I need to calculate my endotoxin limit. My excipient is 0.1% final product and the drug concentration is expressed in vp/mL....

20 January 2021 1,262 1 View