strange derivative curves with PCR HRM?

07 April 2023 0 2K Report

Dears collegues,

I obtained strange derivative curves (difference between samples) of my PCR HRM : the peacks are angular.

It's a 150bp amplification of ADN from a bacterial communinities with classical HRM program, runned on Piko real thermocycler

Thank you for suggestions

Best regards

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Science method

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