Sample: plant seeds, and ethanolic extraction assisted by ultrasonic used for our samples. Both identifying and quantifying are being targeted.
We have BSTFA but I don't know the protocol of these process, and it's possible to use only BSTFA or I should mix derivative reagents. I need to know all the conditions of the process and if any other chemicals or reagents needed.
thanks in advance
Benzylation, silylation, acetylation, methyl ester formation are usually used for GC-MS derivatization of samples. Acetonitrile can be used for improved solubility. Add 1% trimethylchlorosilane (TMCS) for improved reactivity for your material. A silylating mixture (Merck 85433) can be more useful.
It would greatly assist if you could provide more details regarding the sample(s), sample preparation and the identity of the analytes (polyphenols). Also are you wanting to identify or quantify or both?
Charles H Hocart I have just provided my description with these information, thanks and waiting for your answer
Please look at the following below links which may help you in your analysis:
https://www.tandfonline.com/doi/full/10.1080/10942912.2012.740645
https://www.researchgate.net/publication/285181355_Gas_Chromatography-Mass_Spectrometry_Analysis_of_Polyphenols_in_Foods
https://repositori.udl.cat/bitstream/handle/10459.1/57932/024250.pdf;jsessionid=B904BB76AF5A433632F486A6FF9F36B1?sequence=5
Thanks
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