Mahmood Hashim

5 Questions 5 Answers 0 Followers

Questions related from Mahmood Hashim

Could someone tell me this reason of this problem on SDS-PAGE?

I have a technical problem with SDS-PAGE, the running sample is a protein extract dissolved in 100 mM NaCl solution, and others dissolved in 10mM PBS solution pH 8.0, the protein concentration per...

07 December 2022 2,829 9 View

Does the encapsulated material concentration affect on particle size of vehicle?

If we encapsulate different concentrations of the same material under the same conditions, it can affect the particle size of the capsule

26 October 2022 5,747 2 View

How could I do the derivatization for polyphenols before analyzing on GC-MS?

Sample: plant seeds, and ethanolic extraction assisted by ultrasonic used for our samples. Both identifying and quantifying are being targeted. We have BSTFA but I don't know the protocol of...

07 June 2021 8,072 4 View

Can we use the general hotplate as a source of heating in the Soxhelt method or not ? what is optimal temperature when using n-hexane as a solvent?

there is a problem with the heating part in soxhelt apparatus, so my question can we use the standard hot plate as a source of heating?

06 December 2020 9,488 2 View

How to calculate the quantity of enzyme needed to hydrolyze protein?

I have alcalase enzyme it's activity 10000U/g and I want to use it in hydrolysis whey protein isolate 90% protein. A suspension of 5% (w/v)WPI prepared so I am asking about the quantity of enzyme...

06 October 2020 9,988 3 View