How could I do extracellular recording from a specific area of rat somatosensory cortex, where the neurons code for a specific body part, for example hind limb toe in behaving animals. I could just use single electrode or arrays up to 16 electrodes. How could I find the right area to record?
Embed multi-channel electrodes into the targeted area. There are many publications describing such technique.
You can typically achieve that by
1. referring to the rodent anatomy books and implanting the electrode on the exact site, if the skull is secured within a stereotactic frame
2. Upon insertion, you can verify the position with mechanical stimulation of the limb and recording centrally
the problem is to finding the exact area. somatosensory cortex has been determined anatomically in reference books, but which part is dedicated to proprioceptives from toe? when we want to insert the electride, the rat is anesthetized. doesn't it supress firing of these neurons? maybe applying mechanical stimulus doesn't give rise to fires in somatosensory cortex. does anybody has any experiment?
Oh your drug then needs to change to not inhibit the response. Have you tried the hypnorm and midazolam cocktail? See this paper and I just emailed you a draft manuscript:
doi:10.1038/s41598-017-11349-z
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