How could I analyze cytokinin content in leaf sample by using RP-HPLC?

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Jean Wan Hong Yong

Hi, a review of cytokinin methods is listed here:

https://www.researchgate.net/publication/233324564_Analytical_methods_for_cytokinins?ev=prf_pub

Article Analytical methods for cytokinins

Parthasarathi Theivasigamani

Thank you Mr. Jean Yong

Parthasarathi Theivasigamani, the earlier one is the newer methods using LC-MS. Here is an older paper that we used when we did ELISA.

https://www.researchgate.net/publication/235685998_The_production_of_cytokinins_abscisic_acid_and_auxin_by_CAM_orchid_aerial_roots?ev=prf_pub

Hope it helps?

Article The Production of Cytokinin, Abscisic Acid and Auxin by CAM ...

Ok. Mr. Jean Yong. Its highly useful

Best wishes.

Tomáš Hluska

Check out work of Ondrej Novak, he repeatedly published procedures for cytokinin determination using various HPLC systems

https://www.researchgate.net/profile/Ondrej_Novak/

the other option is Neil Emery https://www.researchgate.net/profile/R_J_Emery/ but I think he uses similar system.

Thank you Tomáš Hluska