I need experimental procedure. Because when I go through literature procedure available by using ELISA.
Hi, a review of cytokinin methods is listed here:
https://www.researchgate.net/publication/233324564_Analytical_methods_for_cytokinins?ev=prf_pub
Article Analytical methods for cytokinins
Parthasarathi Theivasigamani, the earlier one is the newer methods using LC-MS. Here is an older paper that we used when we did ELISA.
https://www.researchgate.net/publication/235685998_The_production_of_cytokinins_abscisic_acid_and_auxin_by_CAM_orchid_aerial_roots?ev=prf_pub
Hope it helps?
Article The Production of Cytokinin, Abscisic Acid and Auxin by CAM ...
Check out work of Ondrej Novak, he repeatedly published procedures for cytokinin determination using various HPLC systems
https://www.researchgate.net/profile/Ondrej_Novak/
the other option is Neil Emery https://www.researchgate.net/profile/R_J_Emery/ but I think he uses similar system.
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