How canI verify the DNA fragment without results after blast in NCBI?

More Qu Tailong's questions See All

How to create ITO materials in FDTD?

Here are the ITO material parameters and the Drude model I found in an article, but I don't know how to import these parameters correctly into Ansys lumerical FDTD.The attached picture shows the...

18 June 2024 3,182 10 View

How to create ITO materials in FDTD?

12 June 2024 5,915 2 View

How to set the background of plot to white color in Silvaco Tonyplot?

Figure 1 is my plot, the background will be colored when the quantity is set to electron concentration. While Figure 2 is the figure i want. How to set the background to white color when the...

11 June 2024 8,621 1 View

How to calculate the number of Zn atoms on the surface of ZIF-8 particles?

I would like to replace the Zn atom on the surface of ZIF-8 particle by the ion exchange. I have the XRD and TEM data of ZIF-8, how many Zn atoms on the surface of this particle?

18 September 2023 5,138 3 View

Regenerative energy system in EV will be a reserch hotspot in the future?

20 August 2023 8,125 1 View

Why is plasmid transfection not effective?

Hello everyone, I am currently doing transient transfection. I want to transfect the overexpressed plasmid into HCC cells, and this plasmid has 10000+ base pairs. The transfection reagent I used...

15 July 2023 3,881 3 View

Is there a solvent that only dissolves Polycarbonate but does not dissolve Poly(propylene carbonate) ?

When I do the PPC-PC reverse dry transfer method, PPC and the sample fall on the PC, and then I melt the PC/ sample /PC onto the silicon chip, how do I remove both PPC and PC to get the sample?

01 July 2023 4,026 1 View

How to simulate 1/f noise in MOSFET with silvaco?

In atlas simulator, i have add interface charge with "interface" keyword, Then i start a noise simulation on this MOSFET with interface charge, but the PSD of 1/f noise shows zero with the...

01 June 2023 4,710 0 View

How to solve equation "xy + ax + by = c" for integer solution of x and y ?

It is well known that the equation in the form of "ax + by = c" (where a, b and c are known integers while x and y are unknown integers) is called linear Diophantine Equation (LDE), and has been...

06 March 2023 1,514 23 View

What does it mean when the relative intensity of XRD peaks changed?

When we synthesized the same compounds at different temperatures, the XRD peaks appeared at the same positions. But the relative intensity of the characteristic peaks changed. For example, the...

06 October 2022 2,950 4 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

Which Scopus Journal provides the most affordable fees?

"PUBLISHING IN A SCOPUS JOURNAL" Researchers are now at a cross road. The critical need to publish in a Scopus or ISI, etc journal is ever vital. Journal Publication fees must be submitted....

10 August 2024 8,620 1 View

Seeking Advice on Viability and Execution of Undergraduate Thesis Topic?

Hello everyone, I am currently developing a thesis proposal and would appreciate your input on its viability and how to effectively carry it out. My proposed topic is: "Does the perceived threat...

10 August 2024 8,992 0 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

Who will be moral responsible for the death of thousands of people in the event of an earthquake?

Who will bear moral responsibility for the deaths of thousands of people in the event of an earthquake? Weeks and months remain before the onset of strong earthquakes that bring death to...

08 August 2024 6,134 12 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Tyler Chafin

What result were you expecting (i.e. what were the primers designed to target)? Are there references present in the NCBI database you searched against which match that target? Which NCBI database did you BLAST against? nt does not include the WGS accessions, so if you are expecting a match there than you might try the RefSeq_genomic database.

Thilina Nethmin De Silva

What primers did you use. Which gene did you amplify?

Qu Tailong

Primers I used for PCV2 gene and the PCR product were sequenced.While BLAST on NCBI, no results.