.
You can separate culture medium from bacteria by centrifugation. After centrifugation, you will have:
- The pellet (bacteria) to extract the DNA or RNA
- The culture medium to measure the concentration of arginine.
Best regards.
Thank you so much sir!
You are welcome!
Articles on" Gender disparities i leatherwork education"
07 August 2024 2,500 0 View
How to get Scopus Author Index ??
18 July 2024 4,080 3 View
I isolated a mixture of three compounds from the stem bark of a plant . I have NMR, Mass spec, IR, and UV data. There are a lot of things I can't make meaning out of. Kindly help me with the...
02 July 2024 9,531 4 View
What is NNFI in confirmatory factor analysis, my LT1 and CFI values are more than .9 can I conclude that model is fit?
30 June 2024 5,598 2 View
I am working on a 21 sample size data time series, and after applying ADF test one of my variables are integrated of order 2 I(2), two variables are integrated of order 1 I(1) and two are...
08 June 2024 5,787 12 View
How does rest promotes,modulates or inhibits the longevity of humans?
07 June 2024 3,964 1 View
Hi, i am currentyl fitting mno2 material into the peak at Mn 2p 3/2 and Mn 2p 1/2. in the right side of the peak, the tip highlighted in red is still not fit enough. is there any idea how to fit...
05 June 2024 7,957 6 View
Hi, Thank you in advance for answering my questions. I am working with a manganese dioxides material with different phase structures (α-MnO2, β-MnO2, and δ-MnO2). I am actually a bit confused...
05 June 2024 9,726 4 View
"Article 51 of the International Code of Zoological Nomenclature (ICZN) (1985) mentioned that when a species' name is cited in a scientific publication for the first time, the author(s) of the...
24 May 2024 545 0 View
Hi, currently i am studying the different structures of MnO2 and i do a BET surface analysis to see the BET surface area. For my synthesized material alpha-mno2 has a BET surface area of 122,...
22 May 2024 3,559 2 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View
I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...
28 July 2024 7,559 1 View
How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?
27 July 2024 6,920 1 View
Gene sequencing related trouble shooting
25 July 2024 4,149 2 View
I found that several expression constructs of published protein structures containing an N-teiminal fusion tag GAMGSGIQRPTST. What's the founction of this fusion tag?
23 July 2024 1,353 4 View
Hi Everyone, I'm using an siRNA kit to knock down a target gene. The kit guarantees that the negative control doesn't target any sequence in mouse genome, and when I use BLAST I don't find any...
23 July 2024 2,673 6 View