Since Triton is a non-ionic detergent, it should not bind to ionexes such as ResourceQ, right? So in principle it should be possible to use ResourceQ chromatography to get rid of excess Triton, right?
I believe you can do it. Using ion-exchange chromatography is quite effective method to exchage the detergents or to remove the detergent. But you have to remember, that there is a chance that a few molecules of Triton can be tightly bound to your sample
It's weird. I've seen anything from no problem to massive peak broadening and smearing apparently being caused by nonionic detergents (which is fixable by dialysis). I'm not sure how this is caused. It' shouldn't be. But it happens. With some proteins there's a problem. With others there isn't.
Thanks for the answers. I've tried it and I got huge peak in the flowthrough where there should be nothing because it was purified previously on Resource Q, so I assume it worked. Of course I do not expect it removed all the Triton, it's just I needed to get rid of excess of the Triton as I had something like 2% concentration doe to concentrating the sample.
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