How can I completely remove DNA in my RNA sample?

12 September 2014 5,927 14 View

Is there a tool for generating probability context free grammar (PCFG) from annotated/ plain corpus?

I am looking for a tool which is source language independent and which can generate PCFG from a plain/annotated corpus.

14 May 2014 8,994 1 View

How to overcome the problem in real-time PCR melting curve variation between the same replicates?

I have started with real time experiments. When I do a melting curve analysis for the primers, I get a different Tm for the same sample in the replicates. But there is no primer dimer, only single...

08 April 2014 9,667 15 View

How to determine the space group of the crystal structure?

There are 232 space groups and 32 point groups. My crystal structure is orthorhombic with pbcn space group. Would you suggest any book or website to determine the space group of crystal structure...

16 October 2013 5,899 7 View

What is the best OCR implementation algorithm?

I need to implement OCR for my project.

11 September 2013 430 4 View

Please help me interpret the following observation in Yeast Signal sequence trap (YSST) assay?trap

Hi, I have done yeast signal trap assay to identify secretary proteins. I have cloned my target gene (only one gene) into YST vector and transformed into invertase mutant yeast. When I culture the...

01 January 1970 9,811 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View