I'm trying to form a complex between a polyphenol and several transition metals. What tests and analysis do I need to verify the complex formation and structure? I'm only using UV-Vis sperto and LC-Ms, is it enough?
Mr. Al-Hafez,
UV, IR, Raman, MS, NMR, SCXRD, EPR (depending on the metal ion), etc....But the shown are basic methods.
Mr. Al- Hafez,
Would you like to mention the polyphenols you are using is pure or extracted from any natural sources. But, if you want only to confirm the formation of polyphenols- Metal complex, try to take NMR of polyphenols before and after formation of complex. You will observe -OH proton shift or even absent.
Mr. Al-Hafez,
according to the valuable answers of the above answerers, please define the structure of your polyphenol (position of the hydroxy group and number of hydroxyl group) and what a kind of metal. You can answer via message if your answer is confidential (only you and me can read your answer).
Please read this link too:
http://www.chem.tamu.edu/rgroup/marcetta/chem104/lectures/104-l-w04.pdf
JRG
Dear
if you want to know the formation of the polypenol - metal complex you can used UV through the clear difference in wave length between the starting material and mixture, LC- MS through the injection of standard polyphenol and mixture the difference indecated the formation of complex .
Thank you all for your valuable answers.
I'm sorry I can't tell which polyphenols I'm working with, but I can tell you the metals; copper, iron (3), and zinc. I did find a clear difference in the UV spectrum between standard and the mixtures. Anyhow, the LC-Ms didn't give me clear results yet. I asked about NMR test but was told that it might not give clear results depending on the matrix of the solutions!
I'm still trying with LC-Ms analysis and would appreciate any reference mentioning the use of Ms to identify a complex!
Thanks
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