How can we store the testicular tissue (not crushed) prior to RNA extraction and for how time we can store?

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How to assess monoamine oxidase levels ?

hello everyone i have a research about monoamine oxidase enzyme and i saw a lot of detection method of it including monoamine oxidase activity assay kit but i don't know what is the easiest and...

18 January 2021 3,474 2 View

Is coronavirus affects children or not?

It has been seen that many deaths of older people happened due to coronavirus, younger was found infected and then recovered yet no any case or less cases or children affected by coronavirus was...

06 December 2020 5,012 18 View

Impact of veil (hijab) and Nimaz (Five times prayers in Islam)??

It was observed globally that by putting mask on face reduced the transmission of coronavirus which is already stated in Islam. Moreover, by praying 5 times a days is essential in Islam in which...

06 December 2020 637 7 View

Anyone knows python??

It is an statistical tool used for data analysis. Anyone have article, abstract or any material regarding to it please share.

30 November 2020 290 5 View

Migratory birds spread diseases?

It has been obsessed since long that millions of birds were migrated every year between the countries and on contenant level. Migration was happened due to seasonal variation, environmental...

30 November 2020 2,897 9 View

How to improve technical writing?

Technical writing is the first and last impression of the researcher. It make the researchers sound and efficient. Some said it is improved by reading research articles while others said it is...

26 November 2020 411 9 View

What is the difference between server, broker, middleware, or gateways used to resolve device level interoperability in IoT?

In order to ensure the device-level interoperability in IoT where two devices with different communication protocols can talk to each other, different solutions exist utilizing either server,...

06 November 2020 9,708 5 View

Can IoT device support more than one communication protocols such as MQTT, CoAP or HTTP etc.?

IoT devices needs a set of communication protocols at different layers such as at the network layer (e.g. IPv4, IPv6), transport layer (e.g. TCP, UDP) and application layer (CoAP, HTTP, MQTT). My...

05 November 2020 5,776 4 View

How we selection column during RPC analysis?

Explain how we choice exact column during reverse phase chromatography

01 November 2020 5,968 14 View

How to use ELISA in the diagnosis of autoimmune disease ?

i searched more about using ELISA to diagnosis autoimmune disease and what i found is that depends on type of the disease. the target will be an antigen specific for the autoantibodies presence in...

25 October 2020 5,502 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Do anyone here can help us in the field of molecular docking?

We have synthesized various metal complexes with different environment around the metals. Now to see the application of synthesized complexes we need this study also. we are also interested in...

01 March 2021 1,899 2 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View