How can we store the testicular tissue (not crushed) prior to RNA extraction and for how time we can store?

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How many grams or milligrams of Artemia should be given to 6 dpf larvae?

Feeding zebrafish larvae properly is a challenge if we need to grow them. According to standard guidelines, how many grams or milligrams of Artemia should be given to 6 dpf larvae?

13 June 2024 389 0 View

Tensile Strength of Cement Mortar ?

Can anybody recommend the tensile strength of cement mortar? Can ASTM C307 be used to test for mortar without fibers?

03 June 2024 7,550 1 View

How measure tolerance factor of BaSrCaTiO3 perovskite?

for ABO3 if only A & B then we used t=rA+Ro/sqrt2(rB+rO) kindly explain for BaSrCaTiO3 ?

31 May 2024 2,036 0 View

what is the relation of macrostrain with particle size?

some data shows direct and some inverse relation? kindly share any authentic paper please.

07 May 2024 6,657 4 View

I need method to prepare silymarin cream ? if available ... precise method with details ?

I need to prepare silymarin cream , I am facing some difficulty with that

06 May 2024 3,614 0 View

Mechanism of copolymer?

Kindlly provide links or literature about the mechanism of free radical copolymerization of styrene and methacrlate, kinetics and rate equation

27 April 2024 404 2 View

What could be the reasons of protein not expressing in Bacillus Subtilis and Bt??

I and using vectors with autoinducibe promoters(Pylb and cyt1). I have performed SDS of 24, 48, 72, 96 and 120 hours. No expression is found in soluble or insoluble form.

14 March 2024 8,752 0 View

3T3-L1 cells are not differentiating after 5 days of induction. What could be the problem?

I induced the preadipocyte after they reached 100% confluency. Then, I treated them with differentiation medium (hg DMEM 10% FBS, 1X P/S, IBMX 0.5mM, Dex 1uM, and Insulin 10 ug/ml) for 2 days....

18 February 2024 8,219 0 View

Deepsoil/Shake 91 multiple motions addtion?

Is there any way to add multiple motion files in a single go to a deepsoil software or to run Shake91 in loop form

30 October 2023 8,858 0 View

Anyone helps me explain textural features in apatite?

The bright zone is high in REEs up to 4% and other zones are depleted in REEs. Can we say overgrowth? But they are enclosed in apatite middle.

25 September 2023 4,667 1 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to Transform my handy-wavy explanation of why group I/II metals oxides in aqueous solution are basic into a rigorous argument?

I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...

03 August 2024 9,918 0 View

Interested in a SCOPUS collaboration?

Hi RG family. My team and I are working on some SCOPUS publications and we need co-authors who are willing and capable of undertaking both qualitative and quantitative-based studies. The scope...

02 August 2024 7,843 0 View