Try Methanol/Water (70/30) for the extraction.
HPLC Conditions :
Column : Zorbax Sb-phenyl, 150x4.6 mm, 5um
Mobile phase : Acetonitrile/Water/Trifluoroacetic acid (200/800/1)
Retention time Sennosides A : 10 min
Retention time Sennosides B: 24 min
sincerely yours.
Thank you Mr. Rachid for the kind advise.
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