How can we purify sennosides from a crude plant extract of Cassia?

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Zakaria Rachid

Try Methanol/Water (70/30) for the extraction.

HPLC Conditions :

Column : Zorbax Sb-phenyl, 150x4.6 mm, 5um

Mobile phase : Acetonitrile/Water/Trifluoroacetic acid (200/800/1)

Retention time Sennosides A : 10 min

Retention time Sennosides B: 24 min

sincerely yours.

Saransh Gupta

Thank you Mr. Rachid for the kind advise.