How can I cultivate MDA-MB-231 cell line?

Hello everybody,I am going to cultivate MDA-MB-231 cell line along with three other cell lines. I want to know if I can cultivate this cell line with RPMI-1640 Medium under the following...

05 June 2016 2,418 8 View

How can I cultivate MDA-MB-231 cell line?

Hello everybody,I am going to cultivate MDA-MB-231 cell line along with three other cell lines. I want to know if I can cultivate this cell line with RPMI-1640 Medium under the following...

05 June 2016 7,492 18 View

Problem with gene expression. Can anyone lend their expertise?

Hello , I'm studying on genes involved in cancer, but lately I expose with some problems. These lately studied genes have very low expression levels and I can hardly detect their expression in...

08 September 2015 1,218 10 View

Can anyone please share a protocol for extracting exosomal RNA from serum?

Hi everyone, I am trying to extract exosomal RNA from serum using ExoQuick kit but I'm not getting any RNA from it, can anyone please tel me how to extract exosomal RNA from serum and is this kit...

31 December 2014 2,897 18 View

Problem with RT-PCR. Can anyone lend their expertise?

07 August 2014 2,555 13 View

How can I isolate WBC for RNA extraction?

Can anyone please share a protocol for isolation of white blood cells and extraction of RNA from these cells (RNA isolation from these cells only)?

03 April 2014 7,223 12 View

How can I isolate a given protein as an intact protein by flow cytometry?

I would like to isolate a given protein as an intact and functional protein by flow cytometry (without protein unfolding or denaturation), can I do that?

03 April 2014 4,463 4 View

Can I get the product of 320bp for real time PCR with SYBR green?

Can anybody please guide me whether I can use SYBR green for the product of 320bp or not? I heard that the best product size is usually less than 200bp ( 80-170 bp), but I think a 320bp product...

03 April 2014 4,981 14 View

How can I isolate plasma for analysis of circulating microRNA?

Can anybody please guide me as to how to isolate plasma for analysis of circulating microRNAs? I would like to isolate plasma immediately after blood collection (with high quality).

03 April 2014 9,115 10 View

Can anyone please share a protocol for the extraction of total RNA using RNAXplus Kit?

I cannot properly isolate sticky cell lines for extraction of total RNA. I am getting low concentration of RNA after extraction (poor band on agarose gel).

02 March 2014 2,817 4 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

How can I assign a velocity along one direction to CO2 molecules in a molecular dynamics simulation using GROMACS?

Dear all, I am trying to perform a MD simulation about the interaction between CO2 and a polymer. I create my model, solvate it and perform the NVT and NPT equilibration. Now I am not able to...

26 February 2021 10,014 3 View

Recalcitrant seed short-mid storage method?

Dear peer scientists, Ultradry storage is not suitable for recalcitrant seeds. However, theoretically, if we can stabilize the structure of the subcellular membrane, as well as glassify the...

25 February 2021 2,442 1 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

Is there a suitable way to confine cell attachment area inside a multi-well plate?

Are there any types of cylindrical cell culture inserts that are designed to confine cell seeding area? Say, if I'm using a 12 well plate (3.5 cm^2/well), but only want to seed cells in the middle...

22 February 2021 7,127 4 View

Are exogenous molecules transferred between human cells during doubling ?

Hello RG community, I wonderer whether exogenous molecules (e.g. reporter plasmids, miRNAs or other artificial molecules) are transferred between cultured human cells during their doubling ? So,...

21 February 2021 6,633 3 View

Has anyone encountered any problems with abm good microRNA primers for quantification of mature miRNAs?

I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...

17 February 2021 8,986 2 View

Is it possible to use maize mature seed to do transformation for maize transgenic study?

Almost all research reports which i ever seen work on Early mature embryo for Agrobacterium mediated maize transformation. In other grass families like rice, we can use mature seed and what do...

17 February 2021 1,434 13 View

Dealing with BMDM seeding and RNA extraction ?

biomedical master student here , i am dealing with mice BMDM and would like some help , i seed 0.5 x 10^6 cells/per well in a 6 well plate but when i extract the RNA i get a very low yeild , i...

15 February 2021 1,102 5 View

Is secondary metabolite production more at vegetative growth stage or during reproductive stage?

Hi, I am working on the effect of heavy metals on a medicinal plant by pot experimentation. Analyzing the effect of heavy metals on secondary metabolites is one of the parameters. I will be...

11 February 2021 6,683 6 View