How can we measure p53, bcl2 and calcium in seminal fluid by flow?

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Rafael Freitas

I don't know about seminal fluid...but for peripheral blood lymphocytes this is a good protocol: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3960957/

Huda Mossa Omran

Thank you Rafael, It is very helpful to me. I wonder if you can help me in calcium measurment. Can we fix or freeze samples and how can we interpret the result s

http://www.nature.com/protocolexchange/protocols/172#/related-articles