Do you fixate the cells for the microscopy? (I mean i guess so, if you use antibodys for staining but better to ask)

If so, you could try finding an antibody against components of the DREAM complex. It is bound to many promoters in G0 and gets released during G2 and replaced ba different proliferation complexes later on.

Hello dear Thomas

Thank you for your response

I am not working in the lab currently, I am writing a review about measuring T cell proliferation using BrdU assay with fluorescence microscope .My question is how I can analyze the results of fluorescence microscope images ?

Hi Usama,

ImageJ is free software that could do the trick.

With kind regards,

Ronald

Hello dear

Many thanks for your answer

Hello

To analyze the results of fluorescence microscope images, Image J is well known software.

Thank you so much dear Masuma

Dear Usama,

I have published this book chapter that might be of your interest. Good luck!

Article DNA Labeling In Vivo: Quantification of Epidermal Stem Cell ...

Thank you so much dear Elisa