How can we make a gene correction of LRRK2 mutation on iPSC reprogrammed from fibroblasts of a parkinsonian patient ?

12 November 2014 1 9K Report

I should correct the LRRK2 mutation on my cells. But I hope to know is it better to do the correction on fibroblasts (before reprogramming them on iPSC) or on iPSC (after reprogramming)? And if there is a protocol published on how to do the gene correction?

Thank you for your help

Nesrine Abboud

Hi Jonas,

Thank you for your answer. The mutation is G2019S of LRRK2 gene. I found 2 publications mentioning ZFN method but they didn't give any details. Could you olease give me companies names which market these isogenic lines? I will also look for collaborations.

Best

Nesrine

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