Why are there 'Us' instead of 'Ts' in 16S rRNA gene sequence?

I have downloaded a sequence of 16S rRNA gene of Staphylococcus aureus from online database SILVA (https://www.arb-silva.de). While the DNA sequence of any gene is supposed to have 'Ts' instead of...

04 May 2019 6,021 5 View

Is it okay to use all block letters for binomial nomenclature?

For writing binomial names of organisms we usually write a capital first letter for genus while all small letters for species. For example: Staphylococcus aureus. What if we are writing all block...

11 December 2018 1,557 6 View

Are MIC values determined on visual inspection of inhibition causing concentration, or on the basis of any specific percentage of inhibition?

I am using Broth micro dilution method on 96 well plates for the determination of MIC values of some of the natural compounds I have isolated. I am using varying concentrations of the compound...

02 March 2018 8,780 7 View

Calculation of percent inhibition of bacteria ?

I am performing antibiotic susceptibility study against S. aureus by broth micro-dilution method using 96 well plates and using MTT dye as a marker of viability. Usually after dispensing MTT dye...

11 December 2017 7,551 5 View

What should be the suitable marker of cellular viability while doing broth micro-dilution method for the purpose of antibiotic susceptibility.

I am intending to perform antibiotic susceptibility assay on Staphylococcus aureus cultures, by broth microdilution method. Now usually Resazurin Sodium salt (Alamar Blue) is used as a marker of...

01 January 1970 9,607 5 View

For how long will the cultures of Staphylococcus aureus remain viable when grown on Mannitol Salt Agar ?

For how long will the cultures of Staphylococcus aureus remain viable when grown on Mannitol Salt Agar ?

01 January 1970 7,312 5 View

What should be the suitable media for antibiotic susceptibility testing by broth micro-dilution method.

I am intending to perform antibiotic susceptibility assay on cultures of Staphylococcus aureus by broth microdilution method, using resazurin sodium dye as a marker of cellular viability. For this...

01 January 1970 2,175 15 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View

I have a problem with kanamycin - why the kanamycin doesn't affect?

Hi everyone, I need to expression of GFP with pET28 plasmid in E. coli BL21(DE3) plysE. Transformed cells have kanamycin and chloramphenicol resistance. Normally, only transformed cells should...

22 February 2021 4,032 4 View

How does elongation affect the resistance of an electrode?

What are the testing rate for electrodes recommended by ASTM for cochlear implants. I am currently doing the 15mm/min elongatio testing.

22 February 2021 1,515 1 View

Resistivity of PVD TiNx thin films vs. N2 flow?

In the PVD of TiN thin films (~100A), I've found the resistivity of the TiN films dropped from ~100 to 60um*cm with N2 flow increasing up to the metallic mode ending point; then the resistivity...

21 February 2021 1,864 2 View

Where can the fault diagnosis data set of lithium battery be found?

Most of the data sets on the network only contain good information about the voltage and internal resistance of lithium batteries. The data of faulty battery is missing. Who can help me, I will be...

21 February 2021 8,659 1 View

Could you please share your experience with me on the type of 96 wells plate for MIC determination: Treated Vs Untreated and Round-Vs Flat-bottomed?

I am working on the MIC determination of various antibacterial agents (both water-soluble and- insoluble) against S. aureus, MRSA, and S. pyogenes. Some guidelines recommend using round-bottomed...

17 February 2021 7,029 3 View

Can 5-FOA Diffuse Through Agar?

I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...

16 February 2021 169 4 View

How to produce huge amount of protoplasts from Fusarium graminearum?

Hi, all I am using some protocol for protoplast production from Fusarium graminearum (Fg), but I need them in a huge amount. Sometimes the protocol worked well but most of the time failed....

14 February 2021 2,286 4 View