Muhammad Amir Khan

8 Questions 4 Answers 0 Followers

Questions related from Muhammad Amir Khan

Why are there 'Us' instead of 'Ts' in 16S rRNA gene sequence?

I have downloaded a sequence of 16S rRNA gene of Staphylococcus aureus from online database SILVA (https://www.arb-silva.de). While the DNA sequence of any gene is supposed to have 'Ts' instead of...

05 May 2019 6,089 5 View

Is it okay to use all block letters for binomial nomenclature?

For writing binomial names of organisms we usually write a capital first letter for genus while all small letters for species. For example: Staphylococcus aureus. What if we are writing all block...

12 December 2018 1,612 6 View

Are MIC values determined on visual inspection of inhibition causing concentration, or on the basis of any specific percentage of inhibition?

I am using Broth micro dilution method on 96 well plates for the determination of MIC values of some of the natural compounds I have isolated. I am using varying concentrations of the compound...

03 March 2018 8,833 7 View

Calculation of percent inhibition of bacteria ?

I am performing antibiotic susceptibility study against S. aureus by broth micro-dilution method using 96 well plates and using MTT dye as a marker of viability. Usually after dispensing MTT dye...

12 December 2017 7,602 5 View

How can we label organisms as sensitive, intermediately resistant and resistant?

Based on the MIC and/or MBC data of antibiotics against any bacteria, how can the organisms be labelled as sensitive, intermediately resistant and resistant?

12 December 2017 6,075 3 View

What should be the suitable marker of cellular viability while doing broth micro-dilution method for the purpose of antibiotic susceptibility.

I am intending to perform antibiotic susceptibility assay on Staphylococcus aureus cultures, by broth microdilution method. Now usually Resazurin Sodium salt (Alamar Blue) is used as a marker of...

01 January 1970 9,653 5 View

What should be the suitable media for antibiotic susceptibility testing by broth micro-dilution method.

I am intending to perform antibiotic susceptibility assay on cultures of Staphylococcus aureus by broth microdilution method, using resazurin sodium dye as a marker of cellular viability. For this...

01 January 1970 2,219 15 View

For how long will the cultures of Staphylococcus aureus remain viable when grown on Mannitol Salt Agar ?

01 January 1970 7,355 5 View