The question is not clear. What are the input parameters and what values are determined from Fluent? Assuming that pressure drop is obtained from Fluent, we can determine the friction factor from the Darcy's definition.
If the pressure drop is obtained from Fluent, we can determine the friction factor from the Darcy's definition. Estimate the average velocity from the velocity profile. The density of the fluid, the tube diameter and length are known. Substituting the values in Darcy equation, the friction factor can be estimated.
it appears you use equations for the estimation of friction factor available in the literature. If the determination of friction factor is a section of a larger analysis, it can be estimated in this manner. If it is fundamental in nature to determine friction factor, It should be determined in the procedure stated earlier.