To silence genes of a particular pathway to study the significance of said pathway.
Go through the link, might be helpful
http://www.ncbi.nlm.nih.gov/pubmed/11740801
The easiest way to "silence" genes would be to use siRNA/shRNA to target the respective RNA of your gene of interest - so called knock-down. These antisense RNAs bind to the RNA of your gene of interest and thus inhibit the expression of the corresponding protein. Depending on the cells your working with you have differnt options for getting the siRNAs into the cells: easy: transfection e.g. via lipofection, harder: retroviral or lentiviral transduction - but you can also buy readymade virus stocks. You may then check the efficiency of your knock-down (it's rare to achieve a 100% knock down) by western blot with antibody against you protein (gene product) of interest. Hope that helps.
Thanks for suggestions
Is it possible in in-vivo conditions. Can we inhibit a particular pathway in not only a particular organ but also in a whole organism (rat or mice)
what you are looking for is knock-out mice. Genes will be "deleted" from the genome of mice. However, targeting essential pathways/genes will be lethal to mouse embryos.
It is better to explain with example. If we have one disease in model rat with different altered pathways. Now For study of particular pathway effect on the disease state, better to silence the whole pathway (by important receptor or protein or signaling molecule) and then can be find alternate strategy. Like in heart, we identified 3 pathways, now how can we further explored which one is the more effect during disease for inventing therapeutics.
This a question in which pharmacology is useful. There are lots of compounds which are good inhibitors for use in vivo, depending of what you want to test. A lot of new samll molecules that inhibit EGF receptor signaling in tumor cells is a good start to understand it
Different Drugs have different abilities to activate or inhibit particular signaling pathways. In the attached paper you can see an example
Article Heat Shock Activation of Phospholipase C Signaling Pathway i...
Another example
Article Heat Shock Activation of Phospholipase C Signaling Pathway i...
Hi, pharmacological inhibitors should be useful and very efficient for this kind of studies.
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