Can anyone want to share method for PCA plotting or suggest a free tool for PCA analysis of proteomic data?

More Dr. Narendra Kumar Sharma's questions See All

Anyone can help with endnote style for shock?

I tried to search output style for shock journal but didnt find. If any one want to share style or information about same style reference of other journal.

10 November 2015 5,120 1 View

Anyone have idea how to find a miRNA target site?

I wants to find miRNA target site by manual or tool available foe access

08 September 2014 1,803 16 View

Can anyone suggest a reliable tool for the design of qRT-PCR primers when used with sybr green?

Can any body suggest or share idea about primer design for qRT-PCR for gene Perilipin 2 step by step for beginner?

04 May 2014 5,498 10 View

How do you make a ROC curve from tabulated data in R?

I want to make an ROC curve from tabulated data using R. Can anyone share the codes or any tutorial for doing this?

02 March 2014 7,223 3 View

Is it possible to identify different oxidized or reduced state of a protein in mixture by mass spectroscopy?

I want to study a signaling molecule which shows reduced or oxidized nature.

01 February 2014 7,013 4 View

Which methods is best for comparing the Plasma proteome between two groups by LC-MS?

I have plasma samples and want to compare two groups to identify differentially expressed proteins by LC-MS. There are several approaches like label free and iTRAQ. I want to know which method is...

01 February 2014 3,401 11 View

Is it necessary to acetone precipitate plasma proteins before iTRAQ labelling for mass spec?

As Plasma has high amount of salt and lipids, what is the best methodology for sample preparations before labelling?

31 December 2013 8,783 3 View

How can we inhibit a particular signaling pathway?

To silence genes of a particular pathway to study the significance of said pathway.

31 December 2013 9,811 9 View

Is there any free software to make hierarchical clustering of proteins and heat maps with expression patterns?

31 December 2013 1,381 11 View

Is a cut-off filter suitable to exchange buffer from protein samples?

I experienced, that when I used 3 or 5 kd cut-off filter tubes to exchange buffer for proteomics work, the flow through have some protein content. Theoretically it should not have happened. In...

31 December 2013 7,753 3 View

Request Python code?

Request Python code from this article : Gender equity of authorship in pulmonary medicine over the past decade. THANKS!

08 August 2024 6,242 2 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

Why does everyone use vs code?

Visual Studio Code (VS Code) has become a popular choice among developers for several reasons: 1. **Free and Open Source**: VS Code is free to use and open source, making it accessible to...

07 August 2024 7,013 4 View

Is an invitation to join the editorial board of Clinical Cardiology Updates a scam?

I received an e-mail invitation to join the editorial board of Clinical Cardiology Updates. While I have published a few articles related to cardiovascular disease, there are lots of colleagues...

06 August 2024 8,981 8 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

How can i do multivariate Time Series forecast using MLP, ANFIS and LSTM?

I need the python code to forecast what crop production will be in the next decade considering climate and crop production variables as seen in the attached.csv file.

05 August 2024 2,977 3 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

Fabian Coscia

Hi,

I can highly recommend Perseus: http://www.perseus-framework.org

It is free and made for proteomic data. PCA is also possible.

Dr. Narendra Kumar Sharma

Dear Fabian

I found the persues but couldn't find the user manual. If possible, please suggest me the route for manual.

you could watch the MaxQuant summer school youtube videos. Some are called Perseus and statistics. The tutorials should be better than a manual.

I tried but the video quality is not good enough to see the words