the product. So I need to cut the band and digest the gel then extract the protein for MALDI or ESI. Also I
In-gel digestion and extraction of digested peptides is a common procedure (in contrast to extraction of proteins).
In our lab, we use the following protocol
http://pel.caltech.edu/protocols/In-geldigestion.html
The washing procedures in this protocol ensure proper desalting as well.
1. Cut the gel band from your PAGE Gel,
2. Excise the gel into as many pieces as possible,
3. Transfer gel pieces into clean centrifuge tube/vial containing water or buffer of your interest (make sure the buffer and concentration u used doesn't interfere with MALDI/ESI), keep in a gel rocker for 4h to overnight.
4. Centrifuge to settle down gel pieces and pipet out supernatant which contains protein. (If u want u can concentrate protein using membrane filtration)
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