Shubhendu Palei

4 Questions 6 Answers 0 Followers

Questions related from Shubhendu Palei

How to remove the interaction of a protein (with high pKa) with E.coli cell membrane ?

Hello everyone, Has someone experienced before, unspecific electrostatic interactions of a protein with high pKa (8.8) with E.coli cell membrane? I am using bacterial display of E.coli for peptide...

01 January 2017 9,544 3 View

If acetonitrile can be converted to acetic acid in acidic hydrolysis conditions, then how is it widely used as a solvent for HPLC in a low pH?

In principle ACN should also hydrolyse in HPLC conditions, but it does not ! Is it not very strange and contradicting ??

08 August 2013 7,547 0 View

How can we extract a protein from SDS-PAGE gel band with out tryptic digestion? I want to do MALDI

the product. So I need to cut the band and digest the gel then extract the protein for MALDI or ESI. Also I

06 June 2013 9,407 2 View

Does anybody have an idea of how to purify hydrophobic peptides by HPLC which are hard to solublize in initial HPLC conditions?

My peptide is hard to soluble in acetonitrile/water/0.5 % TFA solvent for preparative HPLC purification. I also tried in 2 mM NH3, but still it is very less soluble.

05 May 2013 8,215 21 View