some E. coli pathotypes strains have acquired various putative virulence factors in their environment.
it is either you sequence your isolates or screen them against already known virulence genes using PCR
by serotyping, virulence gene profile, and by specific PCR markers
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There have been several virulence signatures to differentiate E. coli pathotypes like EPEC, ETEC, UPEC, EIEC, EHEC. This can be performed by PCR, genome sequencing, oligonucleotide spotted assay. The functionality studies can be done by cell invasion assays, growth kinetics and infections studies also by screening of particular toxins.
Article Differentiation of Escherichia coli Pathotypes by Oligonucle...
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