How can we use immunohistochemistry for quantitative analysis of peptides expression ?

Is there any convenient and relevant method for quantitative use of immunohistochemistry with high accuracy?

06 July 2018 5,882 4 View

How do we can induce Acetycholinesterase over-expression in mice ?

I need a pharmacological protocol to induce acetylcholinesterase over-expression in mice brains, in order to use the brain homogenate as enzyme solution.

07 August 2016 9,832 2 View

Is there any simple procedure for Polysaccharide extract desalination ?

I would like to eliminate NaCl used in the extraction procedure that precipitate while precipitating polysaccharides with ethanol.

04 May 2016 6,891 0 View

Do antioxidant enzymes increase or decrease in vivo under oxidative stress?

I have found many disparencies in literature data. Some authors decribe increased levels of antioxidant enzymes (SOD, CAT, GSH, etc) under diabetes or inflammation, while others reported inverse...

11 December 2015 2,384 12 View

What about alpha amylase concentration for the enzyme inhibition test?

My stock solution is about 12000 U/mL in relation to plant extract what concentration of alpha amylase is recommended and can represent the in vivo conditions ?

10 November 2015 9,335 8 View

Why fresh Food smells good and deteriorated Food smells bad ?

How we can explain that from a neurochemical point of view ?

01 January 1970 1,584 0 View

Collaboration in Enzyme inhibitory Docking ?

Question Link : https://www.researchgate.net/post/Is_any_one_with_expertise_in_molecular_docking_interested_to_join_a_collaborative_work_on_enzyme_inhibitors

01 January 1970 6,344 1 View

Can Food Be considered as a medical treatment ?

01 January 1970 2,138 4 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Energy minimization in LAMMPS using NVT ?

How do I do energy minimization in lammps with NVT ensemble ? I am using the following command and it does not seem to work. It stops at extremely high energy and gives me the error zero search...

01 March 2021 2,294 3 View

Can polymer concentrations be calculated based on the number average molecular weight?

Hello, I spin-cast two different polymers onto wafers and measure their height as a function of initial concentration. For polymer A, I have the weight-average molecular weight (Mw) as well as...

28 February 2021 7,882 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

What is Flat Lens Antenna?

28 February 2021 5,433 3 View

Can impedance of antenna be less than 50 ohms?

I am simulating helix antenna in hfss, right now, giving excitation with 50 ohms port. When I see the results of Z parameter (real). It shows lower than 50 ohms. Do not understand, if it can be...

27 February 2021 4,218 7 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

How to measure angle between laser travel path and machine axis in Laser Measurement system?

25 February 2021 1,987 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View