is there any formula to calculate velocity from absorbance ?
Is there any convenient and relevant method for quantitative use of immunohistochemistry with high accuracy?
06 July 2018 5,973 4 View
I need a pharmacological protocol to induce acetylcholinesterase over-expression in mice brains, in order to use the brain homogenate as enzyme solution.
07 August 2016 9,954 2 View
I would like to eliminate NaCl used in the extraction procedure that precipitate while precipitating polysaccharides with ethanol.
04 May 2016 6,998 0 View
I have found many disparencies in literature data. Some authors decribe increased levels of antioxidant enzymes (SOD, CAT, GSH, etc) under diabetes or inflammation, while others reported inverse...
11 December 2015 2,498 12 View
My stock solution is about 12000 U/mL in relation to plant extract what concentration of alpha amylase is recommended and can represent the in vivo conditions ?
10 November 2015 9,437 8 View
How we can explain that from a neurochemical point of view ?
01 January 1970 1,677 0 View
Question Link : https://www.researchgate.net/post/Is_any_one_with_expertise_in_molecular_docking_interested_to_join_a_collaborative_work_on_enzyme_inhibitors
01 January 1970 6,442 1 View
How relevant are Foods in medical treatment and is there any clear definition for medicinal plants ? Legislation for medicinal products, is not completely harmonized. According to current...
01 January 1970 2,242 4 View
Hi, I'm currently working on a project where I need to plot the atom-projected band structure using GPAW. I've been able to calculate the band structure for my material, but I'm having trouble...
07 August 2024 269 3 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Hello! I have this scale which had 10 items initially. I had to remove items 8 and 10 because they correlated negatively with the scale, and then I removed item 9 because Cronbach's alpha and...
01 August 2024 4,606 7 View
I've seen articles that primarily focus on alpha and beta activity in the frontal regions, but these studies often compare healthy subjects with those having various pathologies. I haven't seen a...
31 July 2024 7,259 1 View
I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...
31 July 2024 193 4 View
Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...
30 July 2024 2,867 0 View
I want to choose a resarch topic regarding enzyme inhibition. So I did my research and found out most of the diseases that originate from enzymes were actually caused by the "deficiency" of...
30 July 2024 2,483 5 View
Dear Researchers, Is it feasible to distinguish between alpha and alpha prime martensite in the Ti6Al4V alloy under rapid solidification in the as-cast state using XRD, in addition to...
30 July 2024 3,849 0 View