How do we can induce Acetycholinesterase over-expression in mice ?

I need a pharmacological protocol to induce acetylcholinesterase over-expression in mice brains, in order to use the brain homogenate as enzyme solution.

07 August 2016 9,832 2 View

Is there any simple procedure for Polysaccharide extract desalination ?

I would like to eliminate NaCl used in the extraction procedure that precipitate while precipitating polysaccharides with ethanol.

04 May 2016 6,891 0 View

Do antioxidant enzymes increase or decrease in vivo under oxidative stress?

I have found many disparencies in literature data. Some authors decribe increased levels of antioxidant enzymes (SOD, CAT, GSH, etc) under diabetes or inflammation, while others reported inverse...

11 December 2015 2,384 12 View

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is there any formula to calculate velocity from absorbance ?

11 December 2015 5,560 0 View

What about alpha amylase concentration for the enzyme inhibition test?

My stock solution is about 12000 U/mL in relation to plant extract what concentration of alpha amylase is recommended and can represent the in vivo conditions ?

10 November 2015 9,335 8 View

Why fresh Food smells good and deteriorated Food smells bad ?

How we can explain that from a neurochemical point of view ?

01 January 1970 1,584 0 View

Collaboration in Enzyme inhibitory Docking ?

Question Link : https://www.researchgate.net/post/Is_any_one_with_expertise_in_molecular_docking_interested_to_join_a_collaborative_work_on_enzyme_inhibitors

01 January 1970 6,344 1 View

Can Food Be considered as a medical treatment ?

01 January 1970 2,138 4 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View