How can we use immunohistochemistry for quantitative analysis of peptides expression ?

Is there any convenient and relevant method for quantitative use of immunohistochemistry with high accuracy?

06 July 2018 5,882 4 View

How do we can induce Acetycholinesterase over-expression in mice ?

I need a pharmacological protocol to induce acetylcholinesterase over-expression in mice brains, in order to use the brain homogenate as enzyme solution.

07 August 2016 9,832 2 View

Is there any simple procedure for Polysaccharide extract desalination ?

I would like to eliminate NaCl used in the extraction procedure that precipitate while precipitating polysaccharides with ethanol.

04 May 2016 6,891 0 View

Do antioxidant enzymes increase or decrease in vivo under oxidative stress?

I have found many disparencies in literature data. Some authors decribe increased levels of antioxidant enzymes (SOD, CAT, GSH, etc) under diabetes or inflammation, while others reported inverse...

11 December 2015 2,384 12 View

No title

is there any formula to calculate velocity from absorbance ?

11 December 2015 5,560 0 View

What about alpha amylase concentration for the enzyme inhibition test?

My stock solution is about 12000 U/mL in relation to plant extract what concentration of alpha amylase is recommended and can represent the in vivo conditions ?

10 November 2015 9,335 8 View

Why fresh Food smells good and deteriorated Food smells bad ?

How we can explain that from a neurochemical point of view ?

01 January 1970 1,584 0 View

Can Food Be considered as a medical treatment ?

01 January 1970 2,138 4 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

Do anyone here can help us in the field of molecular docking?

We have synthesized various metal complexes with different environment around the metals. Now to see the application of synthesized complexes we need this study also. we are also interested in...

01 March 2021 1,899 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View