How can we calculate the transformation efficiency if we transform a callus via Agrobacterium, with patches of GUS positive cells over part of callus?

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Markus Sack

this has been discussed before see and you may find what you need by looking at:

https://www.researchgate.net/post/How_to_calculate_transformation_efficiency_in_plant_transformation

Raham Sher Khan

If the callus is compact, the No. of blue spots can be counted per callus. If the callus is friable, the No. of blue spots can be counted per 100 mg callus, and so transformation efficiency can be calculated.

Atif Iqbal

Thanks, Raham Sher Khan.