How can we calculate the swim speed and swim depth of fresh water reptiles?

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Is it okay to grow bacteria in 30 for cloning? What is the lowest mass of Vecor I can take for cloning? what should be the optimum insert ratio?

I am using a vector that has repeat sequences, and using Mach1 competent cells. I heard that if the cells are not stable cells, then we shouldn't grow them in 37 to avoid recombination to happen...

26 June 2024 3,735 3 View

What is the the target displacement for a retrofitted model outside US conditions (preferably Indian conditions)?

Dear Members of ResearchGate, I hope you are doing well. I have been retrofitting using the ASCE 41 2017 standard. In it, I have two basic doubts. 1. The target displacement as per clause...

13 June 2024 2,773 0 View

What is the best book on seismic retrofitting reinforced concrete structures, preferably for Indian and US conditions (Recommendations please)?

Hello Everyone, I hope you are doing well. What is the best book on seismic retrofitting reinforced concrete structures, preferably for Indian and US conditions (Recommendations please)? Thank...

30 May 2024 9,200 9 View

Is there a way to map gene expression data from real-time PCR on gene trees?

I am trying to understand how the expression of paralogues changed over time. I have real-time PCR data and gene tree. I want to reconstruct the estimated gene expression level at each node. I...

14 March 2024 8,131 0 View

Please can anyone suggest me how to write a book chapter in Elsevier?

21 February 2024 3,672 2 View

What is the best tool or platform for planning research works (Recommendations, please)?

Hi Everyone, I want to know some of the best tools or platforms for planning research works (PhD or/and Master's research). Till now I have been using MS Excel to schedule my work, but it is not...

15 January 2024 6,985 3 View

I have resuspended lyophilised Amyloid beta protein in Guandine HCL, how should I store it for long term use?

We are purifying the protein from recombinant DNA

05 January 2024 1,086 2 View

What are the values of electronic properties of Niobium Nitride (NbN)?

Can someone please help me by providing the values of electronic properties (bandgap,electron and hole density of states, dielectric permittivity and electron and hole mobilities) of Niobium...

02 January 2024 3,962 1 View

In Opensees, why reinforced concrete beam-column joint is assumed as a parallelogram?

Hi everyone. I wish you all a Merry Christmas and a Happy New Year ahead! I hope you are doing well. I have a question. In Opensees, why reinforced concrete beam-column joint is assumed as a...

25 December 2023 3,540 3 View

Why does the absolute value of the negative peak increase at the time of 50 min? Does the binding mode has changed?

A micellar solution of protein (in PBS, pH 7.4) was loaded into the sample cell of the calorimeter. The ligand was prepared in 10% DMSO and was loaded in the injector-stirrer syringe. I have also...

22 August 2023 3,630 16 View

Is it possible to plot the atom-projected band structure using GPAW?

Hi, I'm currently working on a project where I need to plot the atom-projected band structure using GPAW. I've been able to calculate the band structure for my material, but I'm having trouble...

07 August 2024 269 3 View

Swimming/space travel depends on the proprioceptive muscle spindles?

When the entire neocortex is ablated in rodents, although they are still able to swim, all the limbs move continuously and asynchronously (Vanderwolf 2006; Vanderwolf et al. 1978). Normal animals...

03 August 2024 835 3 View

I need help with Identification (misidentification) of European Pisidiidae & Sphaeriidae?

Hi everyone! I published an article summarizing my first experience in determining cryptic species of small bivalves, of course with errors. I will be very grateful and will thank you in future...

02 August 2024 6,582 11 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 366 3 View

Which filtration method to go for run off water from dirty solar panels to be used again?

We are working on a robot that cleans solar panels using fresh water supply and a rotating brush. We are trying to conserve as much water at possible by recycling the dirty water that is collected...

28 July 2024 5,778 2 View

In passive sampling, can polymer-lipid partition coefficients be applied independent of environmental salinity?

For example, if low-density polyethylene (LDPE) is equilibrated with PCB contaminated marine and freshwater sediments, can LDPE-lipid partition coefficients be used to obtain equilibrium...

28 July 2024 263 1 View

Why Orca calculations take too loong?

Hello! I am trying to calculate the sumatriptan molecule, but it has been counting for five days and it does not stop. It seems to me that it is too long and I am doing something wrong. Please...

25 July 2024 3,364 5 View

MDCI module in Orca software?

Dear Researchers, My question is associated with the "MDCI" method in Orca. Please let me clarify my question using a simple example: Suppose we are going to perform CBS extrapolation using "!...

21 July 2024 1,632 0 View

Should a defect induced structure be optimized in a DFT calculation?

I'm trying to study the effects of introducing vacancy defects in my material. If I optimize my structure and find the lattice parameter before introducing vacancy, should I again optimize my...

18 July 2024 1,794 2 View

What is best practice for conducting narrative synthesis for systematic literature review?

Hi everyone, I have found myself in the rabbit hole of attempting to identify a robust method of conducting a narrative synthesis for my protocol. I am reading a variety of methods in both design...

17 July 2024 8,082 3 View