I want to block ROS production in mice and, therefore, looking for probable inhibitory compounds/protocols. I would appreciate it if anybody shares his/her knowledge of this.
Hey Sourodip,
there are studies that used the global and unspecific ROS scavenger NAC in vivo to investigate the role of ROS in their settings. A few examples with doses and duration of treatment are these:
doi: 10.4161/23328940.2014.984556
doi: 10.3892/ol.2018.8864
doi: 10.1046/j.1365-2249.2000.01373.x
doi: 10.1016/j.bbr.2006.06.030
NAC scavenges both extracellular und intracellular ROS and does not differ between different ROS supsecies. So you will not get an answer of the location of ROS production or the ROS sources of your cells in the body you are interested in. This will only tell you, if ROS are involved in your setting or not.
All the best and stay healthy,
Marc
Hello Marc Herb
I really appreciate the clarity with which you answered my query. I quickly went through your links and found them very useful. Thanks once again.
Wishes of good health
Sourodip
In context of this topic, I would like to bring to your attention a recent review of our group, "Functions of ROS in macrophages and antimicrobial immunity".
In this review, we give an introduction to ROS and their sources in macrophages, summarize the versatile roles of ROS in direct and indirect antimicrobial immune defense and provide an overview of commonly used ROS probes, ROS source inhibitors and ROS scavengers (also the difference between ROS scavengers and antioxidants, which are not synonymous, is explained).
If you like, please have a look at:
Functions of ROS in Macrophages and Antimicrobial Immunity
February 2021, Antioxidants 10(2):313
DOI: https://doi.org/10.3390/antiox10020313