After we do the last step of flow cytometry in ki67 assay , how can we interpret the results in term of T cell proliferation ?
Ki67 expression correlate with a proliferative condition.
So, first of all, because this is a intranuclear staining, you need an unlabeled control (to exclude Ab aspecific interactions and cellular auto-fluorescence).
After that, you have to gate on your cellular population and you must be sure that in every samples (unlabeled, unstimulated and stimulated, for example) there are THE SAME photomultiplier tube (PMT) voltages and compensation matrix.
FInally, you can show your data as SSC/Ki67 or overlay Ki67-histograms of your different conditions.
To know if your data is statistically significant, you can use a stastitic programm, such us Prisma.
Good Luck!
Hello dear
I have to write a review on Ki67 assay to measure T cell proliferation.
After we apply flow cytometry , we make diagram represents the Ki67 (logarithmic mode) and propidium Iodide (linear mode) , so how can we use this diagram image to understand T cell proliferation ?
Ki67 expression correlate with a proliferative condition.
So, first of all, because this is a intranuclear staining, you need an unlabeled control (to exclude Ab aspecific interactions and cellular auto-fluorescence).
After that, you have to gate on your cellular population and you must be sure that in every samples (unlabeled, unstimulated and stimulated, for example) there are THE SAME photomultiplier tube (PMT) voltages and compensation matrix.
FInally, you can show your data as SSC/Ki67 or overlay Ki67-histograms of your different conditions.
To know if your data is statistically significant, you can use a stastitic programm, such us Prisma.
Good Luck!
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