How does one interpret polynomial quadratic regression of two variables ?

01 February 2019 4,995 8 View

How to carry out quality control using Trimmomatic on SRA reads of SAGE-RNASeq?

I have carried out a FASTQC analysis on raw reads of SAGE-RNASeq experiment from SRA database. Numerous overrepresented sequences have turned up pertaining to Illumina single end adapters and...

11 December 2016 8,201 0 View

Improving RAM usage of GNU parallel blast?

Running a GNU parallel blast uses only ~2% of my memory per core. My system has a quad-core 24 GB RAM. 1.Can I affect the speed of blast process and reduce the time of run by alloting more memory...

08 September 2016 8,304 2 View

How can I use a proxy with authentication details for R or Rstudio?

I am using R 3.2 and Rstudio in Windows 7. Since, I am using a proxy internet connection, I always get the 407 proxy authentication error. I have tried setting environment variables with my proxy...

01 February 2016 6,558 2 View

Computational requirements to assemble a small mammalian genome (~600 Mb)?

Please provide a minimal advisable requirement of RAM and CPU Cores. Also, provide the time (in hours or days) required per such assembly, if experienced.

01 February 2016 8,591 9 View

Is it possible to predict long non-coding RNAs without a reference genome?

01 February 2016 2,496 2 View

What are the most intriguing questions in the field of non-coding RNA studies?

The field of non-coding RNA research is one of the quickly emerging areas in the world of science. Few years ago, we had just tRNA and rRNA; then suddenly we had ribozymes and now we have the...

09 October 2015 7,131 1 View

How do grid cells get the message that the individual has ventured into a new location in space?

How does a grid cells get the information?

01 February 2015 5,411 4 View

Can anyone help me set up a high performing cluster running on Ubuntu Linux in my laboratory with 128 Gb RAM, 32 Core processor and 1 TB memory?

I have two Quad core HP Z400 workstations and two quad core Acer Veriton series with i3 processor.

04 May 2014 5,863 16 View

What are the basic computing skills needed to learn Next-Generation sequencing?

Linux, Perl, C or C++; are these important to ace before learning sequence analysis in NGS?

02 March 2014 1,313 9 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

How to define tie constraint between rigid body and another part in dynamic explicit model of Abaqus?

Hi dear researchers I connected a rigid body into the part of my structure by tie constraint and then I added displacement to rigid body, in static general step every thing is fine and these two...

28 February 2021 3,442 2 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

What is the difference between genotype and haplotype data in the ped format?

Hi everybody In the ped format for genotype, alleles of any SNP are represented by two columns (one for each allele, separated by a space). Is a column sufficient for the haplotype to...

27 February 2021 1,965 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

What program can I use to caclulate dipole moment efficiently and without the need of excessive computation?

Hi, I am a third year chemistry undergrad doing my group project in which I need to predict the HPLC retention time of 20 aromatic molecules. to do this we require the chemical properties of these...

24 February 2021 1,384 3 View