How does one interpret polynomial quadratic regression of two variables ?

01 February 2019 4,995 8 View

How to carry out quality control using Trimmomatic on SRA reads of SAGE-RNASeq?

I have carried out a FASTQC analysis on raw reads of SAGE-RNASeq experiment from SRA database. Numerous overrepresented sequences have turned up pertaining to Illumina single end adapters and...

11 December 2016 8,201 0 View

Improving RAM usage of GNU parallel blast?

Running a GNU parallel blast uses only ~2% of my memory per core. My system has a quad-core 24 GB RAM. 1.Can I affect the speed of blast process and reduce the time of run by alloting more memory...

08 September 2016 8,304 2 View

How can I use a proxy with authentication details for R or Rstudio?

I am using R 3.2 and Rstudio in Windows 7. Since, I am using a proxy internet connection, I always get the 407 proxy authentication error. I have tried setting environment variables with my proxy...

01 February 2016 6,558 2 View

Computational requirements to assemble a small mammalian genome (~600 Mb)?

Please provide a minimal advisable requirement of RAM and CPU Cores. Also, provide the time (in hours or days) required per such assembly, if experienced.

01 February 2016 8,591 9 View

What are the most intriguing questions in the field of non-coding RNA studies?

The field of non-coding RNA research is one of the quickly emerging areas in the world of science. Few years ago, we had just tRNA and rRNA; then suddenly we had ribozymes and now we have the...

09 October 2015 7,131 1 View

How do grid cells get the message that the individual has ventured into a new location in space?

How does a grid cells get the information?

01 February 2015 5,411 4 View

How can we add chemical residues to the pdb structure of an RNA molecule in In silico mode?

I've to add a methyl group to the C-5 of a Uracil base in the 3D structure of an RNA molecule. Please suggest tools to do so.

06 July 2014 2,328 4 View

Can anyone help me set up a high performing cluster running on Ubuntu Linux in my laboratory with 128 Gb RAM, 32 Core processor and 1 TB memory?

I have two Quad core HP Z400 workstations and two quad core Acer Veriton series with i3 processor.

04 May 2014 5,863 16 View

What are the basic computing skills needed to learn Next-Generation sequencing?

Linux, Perl, C or C++; are these important to ace before learning sequence analysis in NGS?

02 March 2014 1,313 9 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to code open-ended questions in SPSS?

Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...

02 March 2021 1,757 4 View