How can the efficiency of a yeast in the fermentation process be quantitatively characterized? Are there certain parameters that can be determined experimentally?
La eficiencia de la levadura en el proceso de fermentación alcohólica, se puede caracterizar por la producción de alcohol etílico, por la velocidad de consumo de azúcares reductores, etc.
In my opinion, at first, you must identify what kind of efficiency are you interested in. The cultivation of yeasts can have two basic aims generally:
i) production of yeast biomass (baker´s yeast, fodder yeast, .......)
ii) production of yeast metabolites
The third possibility is to employ yeast as "a catalyst" for the conversion of various chemical compounds.
When you specify which process utilizing yeast are you interested in, then you can obtain a more detailed answer concerning parameters for evaluation of efficiency.
The efficiency of yeast in the fermentation process can be quantified through the determination of ethanol using high performance liquid chromatography (HPLC).
The recursive least squares algorithm (RLS) is the recursive application of the well-known least squares (LS) regression algorithm, so that each new data point is taken in account to modify (correct) a previous estimate of the parameters from some linear (or linearized) correlation thought to model the observed system. The method allows for the dynamical application of LS to time series acquired in real-time. As with LS, there may be several correlation equations with the corresponding set of dependent (observed) variables. For the recursive least squares algorithm with forgetting factor (RLS-FF), acquired data is weighted according to its age, with increased weight given to the most recent data. No prior 'training phase' is required.
Years ago, while investigating adaptive control and energetic optimization of aerobic fermenters, I have applied the RLS-FF algorithm to estimate the parameters from the KLa correlation, used to predict the O2 gas-liquid mass-transfer, hence giving increased weight to most recent data. Estimates were improved by imposing sinusoidal disturbance to air flow and agitation speed (manipulated variables). The power dissipated by agitation was accessed by a torque meter (pilot plant). The proposed (adaptive) control algorithm compared favourably with PID. Simulations assessed the effect of numerically generated white Gaussian noise (2-sigma truncated) and of first order delay. This investigation was reported at (MSc Thesis):
Thesis Controlo do Oxigénio Dissolvido em Fermentadores para Minimi...
For what purpose you are using yeast in the fermentation process?
You check the the production products of your interest or consumption of substrate (glucose) using HPLC & GC.
I have used yeast for the production BIOETHANOL from anaerobic fermentation of acid pretreated and enzymaticaly saccharified lignocellulosic biomass of wheatstraw. My thesis is availble on my Researchgate page.