How to know the best match of organism 16S rRNA sequence when I have many hits of different species have same Identity, Query Coverage and E-Value ?

I have done 16S rRNA sequencing for two actinobacteria isolates and when I tried the NCBI blast tool, I got hits for about 10 different species which are 100% identity, 100% query recovery and...

25 July 2018 5,958 3 View

No-overlap fluorescent dyes?

are there any two fluorescent dyes that possess no overlap in fluorescence intensity?

03 April 2018 4,496 5 View

Anyone can help please about the oxygen level of fermentation of actinobacteria in the bioreactor?

I'm asking about the optimum oxygenation level or range for bioreactor fermentation of some soil actinobacteria for production of natural products. Thanks in advance

18 March 2018 4,994 7 View

No title

24 September 2017 530 11 View

What are the gold sputtering condition for PLGA nanoparticles in range of 130-200 nm?

I need specifications for gold sputtering of PLGA nanoparticle samples of size ranges from 130-200 nms for SEM analysis namely the number of targets, current and duration of sputtering.

06 July 2017 5,301 0 View

B16F10 stocks from -80 freezer and liquid nitrogen are not growing well when thawed. Any recommendations?

I tried to thaw several stocks from the -80 freezer that date back to one year and others from liquid nitrogen that date back to two years but they die continuously. I thaw them in 6-cm culture...

06 July 2017 7,671 5 View

What is the best antifungal used in isolation of a mix of bacteria and fungi?

I have some environmental samples that contain both fungi and bacteria and I want to isolate only bacteria What is the best way to do that and if I want to add antifungal in the media, what is the...

18 December 2014 8,004 11 View

What is the best plasmid extraction kit for large plasmids?

If I want to extract large plasmids from bacteria (more than 100 kb), what is the best way and best kit to get them in pure and high yield. Please help me urgently if you can Best Regards

10 November 2014 8,279 15 View

What is the best method for identification of actinomycetes biochemically?

I had isolated some isolates suspected to be actinomycetes. What is the best method for identification of them biochemically? Please help if you can Best regards

29 October 2014 7,629 2 View

What is the best media for cultivation of actinomycetes to assist production of biologically active secondary metabolites?

16 October 2014 1,902 16 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Can chitosan alone can act as a reducing agent for Cu(0) nanoparticle sythesis?

[Synthesizing Cu nanoparticles without using chemical reductants]

01 March 2021 8,359 1 View

What are the new 2D nanomaterials were used for Silver nanoparticles stabilization?

In the stabilization of AgNPs are vital in current research interest. However, some of the 2D nano-materials like graphene oxide, MoS2, etc... previously used to stabilize it. But still they shown...

01 March 2021 2,207 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How to check the uv-vis absorption of nanoparticle by surface plasmon resonance?

I try to check the uv-vis absorption of Indium nanoparticle. Now I am measuring in solution, can I measure it in the form of a film?

01 March 2021 930 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Distribution of particles in metal matrix?

How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...

28 February 2021 7,397 8 View

How can I calculate the rdf of water molecules around 'Na' ions that are closest(adsorbed) to the nanoparticle(solute)?

Hi, I want to calculate the radial distribution function for water molecules around the Na+ ions adsorbed on the surface of a spherical nanoparticle using the gromacs trajectory dynamically? For...

28 February 2021 5,650 1 View