How to know the best match of organism 16S rRNA sequence when I have many hits of different species have same Identity, Query Coverage and E-Value ?

I have done 16S rRNA sequencing for two actinobacteria isolates and when I tried the NCBI blast tool, I got hits for about 10 different species which are 100% identity, 100% query recovery and...

25 July 2018 5,958 3 View

How can the concentration of nanoparticles in plasma peak after 5 hours then decline following intravenous administration in mice?

PLGA nanoparticles (200 nm) showed an extravascular-like plasma profile after intravenous administration with peak concentration after 5 h. Then upon conjugation to cRGDyk (which showed no change...

05 June 2018 4,802 0 View

No-overlap fluorescent dyes?

are there any two fluorescent dyes that possess no overlap in fluorescence intensity?

03 April 2018 4,496 5 View

Anyone can help please about the oxygen level of fermentation of actinobacteria in the bioreactor?

I'm asking about the optimum oxygenation level or range for bioreactor fermentation of some soil actinobacteria for production of natural products. Thanks in advance

18 March 2018 4,994 7 View

No title

24 September 2017 530 11 View

B16F10 stocks from -80 freezer and liquid nitrogen are not growing well when thawed. Any recommendations?

I tried to thaw several stocks from the -80 freezer that date back to one year and others from liquid nitrogen that date back to two years but they die continuously. I thaw them in 6-cm culture...

06 July 2017 7,671 5 View

What is the best antifungal used in isolation of a mix of bacteria and fungi?

I have some environmental samples that contain both fungi and bacteria and I want to isolate only bacteria What is the best way to do that and if I want to add antifungal in the media, what is the...

18 December 2014 8,004 11 View

What is the best plasmid extraction kit for large plasmids?

If I want to extract large plasmids from bacteria (more than 100 kb), what is the best way and best kit to get them in pure and high yield. Please help me urgently if you can Best Regards

10 November 2014 8,279 15 View

What is the best method for identification of actinomycetes biochemically?

I had isolated some isolates suspected to be actinomycetes. What is the best method for identification of them biochemically? Please help if you can Best regards

29 October 2014 7,629 2 View

What is the best media for cultivation of actinomycetes to assist production of biologically active secondary metabolites?

16 October 2014 1,902 16 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View