How to know the best match of organism 16S rRNA sequence when I have many hits of different species have same Identity, Query Coverage and E-Value ?

I have done 16S rRNA sequencing for two actinobacteria isolates and when I tried the NCBI blast tool, I got hits for about 10 different species which are 100% identity, 100% query recovery and...

25 July 2018 5,958 3 View

How can the concentration of nanoparticles in plasma peak after 5 hours then decline following intravenous administration in mice?

PLGA nanoparticles (200 nm) showed an extravascular-like plasma profile after intravenous administration with peak concentration after 5 h. Then upon conjugation to cRGDyk (which showed no change...

05 June 2018 4,802 0 View

Anyone can help please about the oxygen level of fermentation of actinobacteria in the bioreactor?

I'm asking about the optimum oxygenation level or range for bioreactor fermentation of some soil actinobacteria for production of natural products. Thanks in advance

18 March 2018 4,994 7 View

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24 September 2017 530 11 View

What are the gold sputtering condition for PLGA nanoparticles in range of 130-200 nm?

I need specifications for gold sputtering of PLGA nanoparticle samples of size ranges from 130-200 nms for SEM analysis namely the number of targets, current and duration of sputtering.

06 July 2017 5,301 0 View

B16F10 stocks from -80 freezer and liquid nitrogen are not growing well when thawed. Any recommendations?

I tried to thaw several stocks from the -80 freezer that date back to one year and others from liquid nitrogen that date back to two years but they die continuously. I thaw them in 6-cm culture...

06 July 2017 7,671 5 View

What is the best antifungal used in isolation of a mix of bacteria and fungi?

I have some environmental samples that contain both fungi and bacteria and I want to isolate only bacteria What is the best way to do that and if I want to add antifungal in the media, what is the...

18 December 2014 8,004 11 View

What is the best plasmid extraction kit for large plasmids?

If I want to extract large plasmids from bacteria (more than 100 kb), what is the best way and best kit to get them in pure and high yield. Please help me urgently if you can Best Regards

10 November 2014 8,279 15 View

What is the best method for identification of actinomycetes biochemically?

I had isolated some isolates suspected to be actinomycetes. What is the best method for identification of them biochemically? Please help if you can Best regards

29 October 2014 7,629 2 View

What is the best media for cultivation of actinomycetes to assist production of biologically active secondary metabolites?

16 October 2014 1,902 16 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

24 February 2021 2,006 2 View