How can secondary metabolites be extracted from fungi, and what techniques and methods can be used to identify these secondary metabolites?

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Aloke Purkait

Dear Youness,

You can go through steam distillation followed by Medium pressure liquid chromatography (MPLC) separation to obtain secondary metabolites.

Regards,

Aloke

İsmail Emir Akyildiz

Folch binary extraction followed by metabolomic analysis using HRMS

Rosephine Enadeghe

Dear Youness

If its mushroom, you can sun dry, pulverized and use water and organize solvent ratio 2:1 for Extraction via maceration or sohxlet and evaporate or freeze dried the solution. You can use any method to detect the presence of various phytochemicals, gcms, hplc etc

Oukhadjou Youness

thank you for your answer