I have prepared 0.8% agarose solution in 50mM calcium chloride buffer. 2ml of 5% SDS solution was added to the agarose solution as surfactant and then, 2 ml of FluoSpheres™ Carboxylate-Modified Microspheres were also added while mixing the solution continuously. I was expecting these fluorospheres be stabilized in agarose solution. However, the confocal imaging shows the agglomeration of the nanoparticles. When I changed the calcium chloride buffer to PBS, ph 7.4, the particles seem to interacting with buffer. I have attached the imaging pictures.
Does anyone know what actually went wrong?
Thanks!
adding everything/mix everything first except adding the agarose.
then adding 2%-3% agarose adjusting to 0.8%.
Dear Junjie Shao , thank you for the suggestions. I used deionized water and adjusted the amount of SDS solution with respect to the amount of fluorospheres, I obtained the homogeneous solution.
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