I have tried by Shake flask method using octanol-buffer and potentiometric titrations.
Shake flask method- Compounds are acridone derivatives and are insoluble in water. Tried to dissolve using co-solvents like methanol and DMSO, but after partition with octanol corresponding compound peaks were not seen in UV spectra.
Alternatively, dissolved 5mg of the compound in octanol- its completely soluble. But after partition taken UV spectra of buffer- compound peaks were not seen.
Potentiometric titrations- Compounds are acridone derivatives, due to strong interaction between carbonyl group and ring nitrogen- not soluble in NaOH and HCl too.
Can anyone suggest any alternative method for log P and pKa determination of the compounds experimentally?
Please consider my recent publication.
Article Ionization States, Cellular Toxicity and Molecular Modeling ...
An alternative is to determine the log P by high performance liquid chromatography (HPLC or UPLC).
See the attached file:
logD/logP at pH=7.4 could be measured using another HPLC approach, so called ElogD method, described in the publication attached. Measurement of pKa could be achieved using Capillary Electrophoresis method. Low solubility compound may require co-solvent addition, which is described in the second publication attached. The method could be performed using single capillary electrophoresis instrument, of course.
Hope that helps,
Article Measurement of Dissociation Constants (pKa Values) of Organi...
Article ElogD o ct : A Tool for Lipophilicity Determination in Drug ...
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