I have a protein sequence that can be cleaved by MMP-9 enzymes. The sequence: KGPRQIT.
From Kridel, S.J. et al., J.Biol.Chem. 2001, 276:20572-20578 I have found the Kcat, Km value and Kcat/Km value. Can the specific activity of the enzyme be found with these values?
Also I want to know if you can calculate how long it will take for a hydrogel cross linked with bis-cysteine pepetide sequence (same one as above) to be degraded by MMP 9 Enzymes?
I have attached the MMP-9 product sheet.
Thank you.