Karthik Ravichandran

5 Questions 3 Answers 0 Followers

Questions related from Karthik Ravichandran

What are the protocols needed to manufacture injectable VEGF-C without a hydrogel delivery vehicle?

I'm looking at injecting VEGF-C into a rat model, specifically into the medial right thigh tissue. I'm going to do this after removing a lymph node. From the literature i have seen so far, most...

30 June 2015 4,773 1 View

What is the effect of the Molecular weight of a crosslinker and crosslinking density on the mesh size of a hydrogel?

I'm trying to develop a hydrogel which can release proteins at a controlled rate. I'm currently using 4 arm PEG maleimide (10 & 20 kDa) and 2 kDa PEG dithiol has the crosslinker via micheal...

10 March 2015 4,192 2 View

What is the stokes radii of VEG-C growth factor?

I'm looking into controlled delivery of VEGF-C via PEG DA hydrogel. I know how to determine the mesh size of the gel but I want to determine the Stokes Radii of VEGF-C. thank you.

16 February 2015 5,939 1 View

How do I trypsin-digest gelatin co-polymerised with polyacrylamide gels?

I'm using a 30% polyacrylamide gel. I'm making a 6% gel with varied % concentrations of gelatin and varied concentration of trypsin. For my prelinimary experiment: 6% gel 0.1% gelatin 0.1%...

02 January 2015 3,948 6 View

How do you crosslink PEG DA hydrogel with degradable linkers?

I want to degrade PEG DA through enzymes. How do I crosslink PEG DA with degradable linkers? Is this a tricky procedure?

02 July 2014 5,487 2 View