11 Questions 5 Answers 0 Followers
Questions related from Karthik Ravichandran
I'm looking at injecting VEGF-C into a rat model, specifically into the medial right thigh tissue. I'm going to do this after removing a lymph node. From the literature i have seen so far, most...
30 June 2015 4,780 1 View
I want to crosslink FITC Dextran - Thiol with PEG - 4arm Maledimide to make a gel. I then want to use dextranase to breakdown the gel for 1 hour. After this how do you remove the excess FITC...
21 May 2015 5,071 2 View
I'm trying to develop a hydrogel which can release proteins at a controlled rate. I'm currently using 4 arm PEG maleimide (10 & 20 kDa) and 2 kDa PEG dithiol has the crosslinker via micheal...
10 March 2015 4,197 2 View
The MW of VEGF-C I'm considering to use is 15.5 kDa from R&D. The VEGF-C is released from PEGDA. How can the coefficent be calculated so one can control the release rate?
17 February 2015 2,122 2 View
I'm looking into controlled delivery of VEGF-C via PEG DA hydrogel. I know how to determine the mesh size of the gel but I want to determine the Stokes Radii of VEGF-C. thank you.
16 February 2015 5,945 1 View
I want to use PEG DA for controlled delivery of VEGF-C.
09 February 2015 2,259 1 View
I'm combining alginate with polyacrylamide. Alginate will be crosslinked with Ca+2, within polyacrylamide. Once I have a gel, I would like to digest small amounts of alginate without any...
09 January 2015 1,322 3 View
I'm using a 30% polyacrylamide gel. I'm making a 6% gel with varied % concentrations of gelatin and varied concentration of trypsin. For my prelinimary experiment: 6% gel 0.1% gelatin 0.1%...
02 January 2015 3,965 6 View
Using 20KDa PEG DA crosslinked with 2000Da PEG Dithol formed via Michael type addition reaction.
12 November 2014 2,293 3 View
I have a protein sequence that can be cleaved by MMP-9 enzymes. The sequence: KGPRQIT. From Kridel, S.J. et al., J.Biol.Chem. 2001, 276:20572-20578 I have found the Kcat, Km value and Kcat/Km...
08 August 2014 2,507 0 View
I want to degrade PEG DA through enzymes. How do I crosslink PEG DA with degradable linkers? Is this a tricky procedure?
02 July 2014 5,493 2 View