Karthik Ravichandran

11 Questions 5 Answers 0 Followers

Questions related from Karthik Ravichandran

What are the protocols needed to manufacture injectable VEGF-C without a hydrogel delivery vehicle?

I'm looking at injecting VEGF-C into a rat model, specifically into the medial right thigh tissue. I'm going to do this after removing a lymph node. From the literature i have seen so far, most...

30 June 2015 4,783 1 View

How do I remove excess crosslinked FITC-dextran in PEG hydrogels?

I want to crosslink FITC Dextran - Thiol with PEG - 4arm Maledimide to make a gel. I then want to use dextranase to breakdown the gel for 1 hour. After this how do you remove the excess FITC...

21 May 2015 5,075 2 View

What is the effect of the Molecular weight of a crosslinker and crosslinking density on the mesh size of a hydrogel?

I'm trying to develop a hydrogel which can release proteins at a controlled rate. I'm currently using 4 arm PEG maleimide (10 & 20 kDa) and 2 kDa PEG dithiol has the crosslinker via micheal...

10 March 2015 4,199 2 View

What is the diffusion coefficient of VEGF-C?

The MW of VEGF-C I'm considering to use is 15.5 kDa from R&D. The VEGF-C is released from PEGDA. How can the coefficent be calculated so one can control the release rate?

17 February 2015 2,125 2 View

What is the stokes radii of VEG-C growth factor?

I'm looking into controlled delivery of VEGF-C via PEG DA hydrogel. I know how to determine the mesh size of the gel but I want to determine the Stokes Radii of VEGF-C. thank you.

16 February 2015 5,946 1 View

How do you determine the isoelectric point of hydrogels such as PEGDA, gelatin and growth factors - speifically VEGF-C?

I want to use PEG DA for controlled delivery of VEGF-C.

09 February 2015 2,261 1 View

What other enzymes can be used to digest alginate other than alginate lyase?

I'm combining alginate with polyacrylamide. Alginate will be crosslinked with Ca+2, within polyacrylamide. Once I have a gel, I would like to digest small amounts of alginate without any...

09 January 2015 1,324 3 View

How do I trypsin-digest gelatin co-polymerised with polyacrylamide gels?

I'm using a 30% polyacrylamide gel. I'm making a 6% gel with varied % concentrations of gelatin and varied concentration of trypsin. For my prelinimary experiment: 6% gel 0.1% gelatin 0.1%...

02 January 2015 3,967 6 View

How do you conduct swelling experiments for PEG DA hydrogels? What are appropriate time intervals to follow?

Using 20KDa PEG DA crosslinked with 2000Da PEG Dithol formed via Michael type addition reaction.

12 November 2014 2,294 3 View

How can I work out the specific activity of MMP-9 enzymes theoretically?

I have a protein sequence that can be cleaved by MMP-9 enzymes. The sequence: KGPRQIT. From Kridel, S.J. et al., J.Biol.Chem. 2001, 276:20572-20578 I have found the Kcat, Km value and Kcat/Km...

08 August 2014 2,509 0 View

How do you crosslink PEG DA hydrogel with degradable linkers?

I want to degrade PEG DA through enzymes. How do I crosslink PEG DA with degradable linkers? Is this a tricky procedure?

02 July 2014 5,495 2 View