Karthik Ravichandran

15 Questions 6 Answers 0 Followers

Questions related from Karthik Ravichandran

What are the protocols needed to manufacture injectable VEGF-C without a hydrogel delivery vehicle?

I'm looking at injecting VEGF-C into a rat model, specifically into the medial right thigh tissue. I'm going to do this after removing a lymph node. From the literature i have seen so far, most...

30 June 2015 4,828 1 View

How do I remove excess crosslinked FITC-dextran in PEG hydrogels?

I want to crosslink FITC Dextran - Thiol with PEG - 4arm Maledimide to make a gel. I then want to use dextranase to breakdown the gel for 1 hour. After this how do you remove the excess FITC...

21 May 2015 5,113 2 View

Does anyone have experience of using 4 arm 2kDa PEG MAL sold by creativepeg?

I'm trying to make a gel using 4 arm 2kDa PEG MAL and 1kDa PEG dithiol (the cross linker). I can make gels using 10 and 20 kDa PEG MAL, but not with 2kDa Peg Mal. Is there a procedure i can follow?

08 April 2015 9,765 0 View

What is the effect of the Molecular weight of a crosslinker and crosslinking density on the mesh size of a hydrogel?

I'm trying to develop a hydrogel which can release proteins at a controlled rate. I'm currently using 4 arm PEG maleimide (10 & 20 kDa) and 2 kDa PEG dithiol has the crosslinker via micheal...

10 March 2015 4,253 2 View

What happens to the stiffness and other mechanical properties of a implanted medical device made out of medical grade Polyurethane?

The medical device is to replace a soft tissue without any load bearings. Looking for general information and direction to where to look for information on the structural changes of thermoplastic...

24 February 2015 4,990 2 View

What is the diffusion coefficient of VEGF-C?

The MW of VEGF-C I'm considering to use is 15.5 kDa from R&D. The VEGF-C is released from PEGDA. How can the coefficent be calculated so one can control the release rate?

17 February 2015 2,166 2 View

What is the stokes radii of VEG-C growth factor?

I'm looking into controlled delivery of VEGF-C via PEG DA hydrogel. I know how to determine the mesh size of the gel but I want to determine the Stokes Radii of VEGF-C. thank you.

16 February 2015 5,990 1 View

How do you determine the isoelectric point of hydrogels such as PEGDA, gelatin and growth factors - speifically VEGF-C?

I want to use PEG DA for controlled delivery of VEGF-C.

09 February 2015 2,322 1 View

How can you make PEGDA or other PEG hydrogel acidics so that they interact with basic VEGF-C?

I want to control the release rate of VEGF-C from PEGDA. in the paper by Hwang et al (2011), Therapeutic lymphangiogensis using stem cells and VEGF-C hydrogel; They used acidic gelatin with...

04 February 2015 7,000 2 View

What other enzymes can be used to digest alginate other than alginate lyase?

I'm combining alginate with polyacrylamide. Alginate will be crosslinked with Ca+2, within polyacrylamide. Once I have a gel, I would like to digest small amounts of alginate without any...

09 January 2015 1,371 3 View

How do I trypsin-digest gelatin co-polymerised with polyacrylamide gels?

I'm using a 30% polyacrylamide gel. I'm making a 6% gel with varied % concentrations of gelatin and varied concentration of trypsin. For my prelinimary experiment: 6% gel 0.1% gelatin 0.1%...

02 January 2015 4,033 6 View

How do you conduct swelling experiments for PEG DA hydrogels? What are appropriate time intervals to follow?

Using 20KDa PEG DA crosslinked with 2000Da PEG Dithol formed via Michael type addition reaction.

12 November 2014 2,334 3 View

How can you convert powered PLGA to pellets which can then extruded to filaments for 3d printing?

Manufactures mostly sell powered versions of PLGA. I want to 3D print with PLGA. How can we convert powered PLGA (and PLLA, PCL) to pellets before extruding to filaments, which then can be used...

06 November 2014 9,962 1 View

How can I work out the specific activity of MMP-9 enzymes theoretically?

I have a protein sequence that can be cleaved by MMP-9 enzymes. The sequence: KGPRQIT. From Kridel, S.J. et al., J.Biol.Chem. 2001, 276:20572-20578 I have found the Kcat, Km value and Kcat/Km...

08 August 2014 2,549 0 View

How do you crosslink PEG DA hydrogel with degradable linkers?

I want to degrade PEG DA through enzymes. How do I crosslink PEG DA with degradable linkers? Is this a tricky procedure?

02 July 2014 5,540 2 View