I'm going to use small RNA sequencing data from GEO available at NCBI for predicting new miRNA's, but the format of datasets at GEO are .soft and I don't know how I can extract small RNA sequences from those files.
Hi,
with R software, maybe you can try the solution below :
http://www2.warwick.ac.uk/fac/sci/moac/people/students/peter_cock/r/geo/
Several articles reports that three target predictor Target Scan, Miranda and PicTar have predicted highest percentage of experimentally validated miRNA targets, but when I looked at their...
08 September 2015 9,362 5 View
For example, I want to know how many reads are there in this experiment available at NCBI SRA database: ERX242531.Thanks in advance
06 July 2015 7,408 13 View
I downloaded all aves' miRNAs from miRbase and when I blast them using BlastX against aves protein database I see that many miRNA sequences perfectly match on protein coding RNAs. But, as I know...
06 July 2015 5,590 3 View
I'm going to create contig from meleafris EST datast, searching in est file shows that both there are both poly (A) and poly (T) sequences in EST file. Is it related to the methods used by scientists?
11 December 2014 8,529 3 View
I want to extract breast cancer differently expressed genes from microarray dataset available at "http://www.ncbi.nlm.nih.gov/geo/". I downloaded dataset from Gene Expression Omnibus (GEO) and the...
05 June 2014 8,934 10 View
Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...
03 March 2021 8,796 3 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...
03 March 2021 1,401 3 View
I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...
03 March 2021 8,920 3 View
dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...
03 March 2021 6,994 5 View
I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.
02 March 2021 4,359 3 View
Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue
02 March 2021 5,433 5 View
If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis
02 March 2021 7,670 3 View
Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !
02 March 2021 5,785 3 View