I have extracted total RNA through tRizol method and have checked on Nanodrop the results i got were ranging from 1.9 to 2.0 .Afterward i have run it on 2% agarose gel for confirmation. So i got the bands below.
can any tell me whether the Total RNa is degraded or not . Additionally i need some recommendations to improve my procedure
Thank you
As far as it appears on gel, the quality looks ok, but for saying something about the degradation, you must perform bioanalyzer analysis and evalute the RIN value. Furthermore, you must mention unit for any value. Just 1.9-2.0 tells nothing without unit.
As far as it appears on gel, the quality looks ok, but for saying something about the degradation, you must perform bioanalyzer analysis and evalute the RIN value. Furthermore, you must mention unit for any value. Just 1.9-2.0 tells nothing without unit.
You need to have an RNA ladder included on your gel so that the size distribution of your bands can be evaluated. That would help you better determine if there is degradation. The stronger bands likely represent rRNA. If the lower strong double bands are your rRNA, then you don't have a trouble with degradation. If the top two double bands are your rRNA, then I would worry about degradation.
Hi, that is my results after silica-spin column based purification from solid tissue (domestic kit). Unfortunately I haven't photo from my trizol attempts, but they was worse ). You can see 2% agarose gel with EtBr staining (in gel). I consider that my photo shows quite good total RNA integrity with 28S and 18S rRNA (bright bands).
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