How can I tighten error bars on Agilent Seahorse XF96 MitoStress Test?

More Nolan J. Beatty's questions See All

My western blots started out well and have become messy without changing the protocol, any thoughts?

When I started doing my Western Blots in February I had clean results, however, as time as progressed they have become messy (using HEK cells and also became messy in tissue). I haven't changed...

12 June 2024 8,642 3 View

Concept analysis Rodgers Evolutionary Model - critical appraisal?

Hi there, when undertaking concept analysis using Rodger's model, is it expected to undertake critical appraisal of the included papers? Are there any recommended tools for this? Papers I have...

22 November 2023 1,474 0 View

Converting wet ppm concentration from a FTIR gas analyser to dry ppm?

I am trying to convert the wet ppm output from an FTIR gas analyser and wanted to check I have got the right conversion calculation below. The analyser measures water vapour as a %. Moisture...

07 November 2023 6,585 0 View

What is the difference between the Lectin and Classical pathway of the Complement system?

The Complement system is considered one of the major mechanism by which it is an effective defense or barrier against initial infection or inflammation. This typically have three pathways and I...

11 September 2022 2,219 1 View

What antibodies can I use to stain for cells undergoing necrosis in zebrafish?

Hello everyone! I am trying to identify cells that have undergone necrosis in zebrafish embryos using IF staining. I saw a paper that monitored necrosis in the eyes of zebrafish by staining RIP1...

25 October 2021 3,928 1 View

We are seeing the plasma in our vacuum system (RF magnetron PVD) flicker on and off at a high frequency?

my group has a small vacuum chamber built for thin film co sputtering, the two guns were initially coupling but we solved that issue by spacing the guns farther apart. However, the issue with the...

22 October 2021 5,692 4 View

Spine surgeons: Do you prefer laminoplasty or lami w/ fusion for cervical spondylotic myelopathy, and why?

Spine surgeons: please see attached study which will be published in Neurospine in 2021 regarding cervical laminoplasty (CLP) vs cervical laminectomy with fusion (CLF) for cervical spondylotic...

08 December 2020 8,142 8 View

Why am I getting a p-value equal to 1 for both Bray Curtis and Jaccard dissimilarity when running a PERMANOVA (adonis function in R)?

Background- I am studying skin bacterial changes in salamanders before and after translocation and have two source populations. I am trying to look at differences in skin bacterial OTUs between...

08 December 2020 1,426 3 View

Maximum concentration of PhMgCl in THF?

The background of this is lengthy, but in short, I am an experienced chemist with essentially no experience doing Grignard reactions. Most recent literature uses mmol scales @ 0.1-1M...

10 October 2020 9,264 13 View

Troubleshooting ddPCR 'rain storm' and low amplitude positives?

I recently ran some ddPCR using probes that have worked beautifully many times and have had two frustrating issues pop up. Firstly, running cDNA from mRNA, the positive population with my gene of...

17 June 2020 8,040 2 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to do Mann-Whitney U test with Bonferroni corrected p-values?

Dear All, My lab primarily works on insect wing patterns. In one of the projects, my student and I have defined 19 abnormality characters on the forewing and 6 abnormality characters on the...

31 July 2024 6,464 5 View

Bonferroni correction. I have independent t-test, paired t-test and ancova conducted. Which test would require Bonferroni adjustment?

I have two groups that I test on three different tasks. I have 4 independent t-test, 6 paired t-test and 2 ANCOVA. My concern for which t-test should I conduct bonferroni correction. At the moment...

28 July 2024 7,827 6 View

Can I use Likert scale with Paired Sample T-test?

Hey researchers! I am currently doing a research about to what extent in which A has accelerated the inclusiveness of the payment system in my country. Down below are a few examples of the...

26 July 2024 5,654 3 View

AGILENT HPLC 1100 SERIES , any expert available please?

AGILENT HPLC 1100 SERIES operation assistance needed.

25 July 2024 10,045 2 View

Paired t-test or unpaired t-test for my quantitative data with SPSS?

I am conducting a qualitative-driven approach to mixed-method research. The role of my quantitative data is to corroborate the findings of the qualitative data. Qualitative data has been collected...

24 July 2024 9,799 3 View

How to transfect circular mRNA to T cells?

Hello! We are using LNP to deliver circular mRNA into T cells. However, compared with linear mRNA, the transfection efficiency and protein expression duration are similar. Are LNP generation or...

21 July 2024 4,322 1 View

How to perform immunofluorescence on suspension cells?

Some time ago, I wanted to perform immunofluorescence on the S protein of mouse CD4 T cells using a Leica super-resolution microscope. I fixed with 4% paraformaldehyde, then added it to the well...

20 July 2024 3,797 2 View

How to activate 4E8 T cells with plate-bound anti-CD3 for a genome-wide CRISPR screen?

It was assumed that 4E8 T cells should be isolated and activated using plate-bound anti-human CD3, after which would be used for a genome-wide CRISPR screen. (Ref: Genome-wide CRISPR Screens in...

17 July 2024 3,371 0 View

Katerina Jerabkova-Roda

Are you using SD or SEM? SEM looks on big graphs like this better.

Nolan J. Beatty

I'm using SD here because there are replicates of 7.

Lillian Onwuha-Ekpete

Hi Nolan,

Have you normalized your data to the number of cells in each well?

How long did you allow for the plate/wells needed to equilibrate to a uniform temp.?